Abstract
Geobacillus thermodenitrificans, with a double-site mutation in l-arabinose isomerase, produced 95 g l-ribulose l−1 from 500 g l-arabinose l−1 under optimum conditions of pH 8, 70°C, and 10 units enzyme ml−1 with a conversion yield of 19% over 2 h. The half-lives of the mutated enzyme at 70 and 75°C were 35 and 4.5 h, respectively.
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Acknowledgments
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2007-D00073) and by the Korea Science and Engineering Foundation (KOSEF) through the National Research Lab. Program funded by the Ministry of Science and Technology (R0A-2007-000-20015-0).
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Yeom, SJ., Ji, JH., Yoon, RY. et al. l-Ribulose production from l-arabinose by an l-arabinose isomerase mutant from Geobacillus thermodenitrificans . Biotechnol Lett 30, 1789–1793 (2008). https://doi.org/10.1007/s10529-008-9746-x
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DOI: https://doi.org/10.1007/s10529-008-9746-x