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Increase in d-tagatose Production Rate by Site-directed Mutagenesis of l-arabinose Isomerase from Geobacillus thermodenitrificans

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Abstract

Among single-site mutations of l-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of d-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size of amino acid 475 were important for d-tagatose production. Among double-site mutations, one mutant converted d-galactose into d-tagatose with a yield of 58% whereas the wild type gave 46% d-tagatose conversion after 300 min at 65 °C.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Sejong University, Seoul, 143-747, South Korea

    Hyo-Jung Oh, Hye-Jung Kim & Deok-Kun Oh

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  1. Hyo-Jung Oh
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  2. Hye-Jung Kim
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  3. Deok-Kun Oh
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Correspondence to Deok-Kun Oh.

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Received 31 August 2005; Revisions requested 27 September 2005; Revisions received 8 November 2005; Accepted 8 November 2005

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Oh, HJ., Kim, HJ. & Oh, DK. Increase in d-tagatose Production Rate by Site-directed Mutagenesis of l-arabinose Isomerase from Geobacillus thermodenitrificans. Biotechnol Lett 28, 145–149 (2006). https://doi.org/10.1007/s10529-005-5327-4

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  • DOI: https://doi.org/10.1007/s10529-005-5327-4

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