Abstract
Over-production of human soluble B lymphocyte stimulator (hsBLyS) was carried out with four different fed-batch culture strategies using lactose as inducer, instead of IPTG, in a fed-batch culture of Escherichia coli. As lactose acted as both inducer and carbon source, the best and simplest culture strategy was direct feeding of lactose after batch culture, thereby giving hsBLyS at 3.7 g l−1 and a productivity of 0.11 g l−1 h−1.
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JY Dai SQ Zhang P Liu (2001) ArticleTitleEukaryotic expression of hsBLyS and screening of expressing conditions Chin. J. Nanjing Normral University (Nat. Sci.). 24 87–91 Occurrence Handle1:CAS:528:DC%2BD3MXmt1Cqtr4%3D
RS Donovan CW Robinson BR Glick (1996) ArticleTitleOptimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter J. Ind. Microbiol. 16 145–154 Occurrence Handle10.1007/BF01569997 Occurrence Handle1:CAS:528:DyaK28XisFSkt7s%3D Occurrence Handle8652113
AK Gombert BV Kilikian (1998) ArticleTitleRecombinant gene expression in Escherichia coli cultivation using lactose as inducer J. Biotechnol. 60 47–54 Occurrence Handle10.1016/S0168-1656(97)00185-5 Occurrence Handle1:CAS:528:DyaK1cXhvVSit7k%3D Occurrence Handle9571801
BG Hall (1982) ArticleTitleTransgalactosylation activity of ebg β-galactosidase synthesizes allolactose from lactose J. Bacteriol. 150 132–140 Occurrence Handle1:CAS:528:DyaL38XhvFChtLo%3D Occurrence Handle6801019
SY Hu JL Wu JH Huang (2004) ArticleTitleProduction of tilapia insulin-like growth factor-2 in high cell density cultures of recombinant Escherichia coli J. Biotechnol. 107 161–171 Occurrence Handle1:CAS:528:DC%2BD2cXos1yl Occurrence Handle14711499
BV Kilikian ID Suárez CW Liria AK Gombert (2000) ArticleTitleProcess strategies to improve heterogeneous protein production in Escherichia coli under lactose or IPTG induction Proc. Biochem. 35 1019–1025 Occurrence Handle10.1016/S0032-9592(00)00137-0 Occurrence Handle1:CAS:528:DC%2BD3cXkslOnsr8%3D
DH Kweon NS Han KM Park JH Seo (2001) ArticleTitleOverproduction of Phytolacca insularis protein in batch and fed-batch culture of recombinant Escherichia coli Proc. Biochem. 36 537–542 Occurrence Handle10.1016/S0032-9592(00)00237-5 Occurrence Handle1:CAS:528:DC%2BD3MXhtVGlsr8%3D
MW Laird GC Sampey K Johnson D Zukauskas J Pierre JS Hong BA Cookseya YL Li O Galperina JD Karwoski RN Burke (2005) ArticleTitleOptimization of BLyS production and purification from Escherichia coli Protein Expr. Purif. 39 237–246 Occurrence Handle1:CAS:528:DC%2BD2MXitFaksA%3D%3D Occurrence Handle15642475
AK Panda RH Khan KBC Rao SM Totey (1999) ArticleTitleKinetics of inclusion body production in batch and high cell density fed-batch culture of Escherichia coli expressing ovine growth hormone J. Biotechnol. 75 161–172 Occurrence Handle10.1016/S0168-1656(99)00157-1 Occurrence Handle1:CAS:528:DyaK1MXmsFajsbY%3D Occurrence Handle10553655
V Roschke S Sosnovtseva CD Ward JS Hong R Smith V Albert W Stohl KP Baker S Ullrich B Nardelli DM Hilbert TS Migone (2002) ArticleTitleBLyS and APRIL form biologically active heterotrimers that are expressed in patients with systemic immune-based rheumatic diseases J. Immunol. 169 4314–4321 Occurrence Handle1:CAS:528:DC%2BD38XnvFGnurw%3D Occurrence Handle12370363
V Saraswat J Lee DY Kim YH Park (1999) ArticleTitleSynthesis of recombinant human interleukin-2 via controlled feed of lactose-complex media in fed-batch cultures of Escherichia coli BL21(DE3)[pT7-G3IL2] Biotechnol. Lett. 22 261–265
K Sunitha YO Kim JK Lee TK Oh (2000) ArticleTitleStatistical optimization of seed and induction conditions to enhance phytase production by recombinant Escherichia coli Biochem. Eng. J. 5 51–56 Occurrence Handle1:CAS:528:DC%2BD3cXitVOju7c%3D
JF Wang ZL Xiu SD Fan (2001) ArticleTitleDetermination of glycerin concentration during the fermentation of glycerin to 1, 3-propanediol China. Ind. Microbiol. 31 33–35
D Woyski JR Cupp-Vickery (2001) ArticleTitleEnhanced expression of cytochrome P450s from lac-based plasmids using lactose as the inducer Arch. Biochem. Biophys. 388 276–280 Occurrence Handle10.1006/abbi.2001.2306 Occurrence Handle1:CAS:528:DC%2BD3MXitlOjtLg%3D Occurrence Handle11368165
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Revisions requested 1 September 2005 and 11 November 2005; Revisions received 7 November 2005 and 4 January 2006
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Li, Z., Zhang, X. & Tan, T. Lactose-induced Production of Human Soluble B Lymphocyte Stimulator (hsBLyS) in E. coli with Different Culture Strategies. Biotechnol Lett 28, 477–483 (2006). https://doi.org/10.1007/s10529-006-0002-y
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DOI: https://doi.org/10.1007/s10529-006-0002-y