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Review: Optimizing inducer and culture conditions for expression of foreign proteins under the control of thelac promoter

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Journal of Industrial Microbiology

Abstract

This review examines factors which influence the expression of foreign proteins inEscherichia coli under the transcriptional control of thelac andtac promoters, and discusses conditions for maximizing the production of a foreign protein using this system. Specifically, the influence of IPTG (isopropyl-β-d-thiogalactoside) concentration, temperature, composition of the growth medium, the point in the growth curve at which cells are induced with either IPTG or lactose, and the duration of the induction phase are discussed.

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  1. Department of Chemical Engineering, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada

    R S Donovan, C W Robinson & B R Glick

  2. Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada

    R S Donovan, C W Robinson & B R Glick

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Donovan, R.S., Robinson, C.W. & Glick, B.R. Review: Optimizing inducer and culture conditions for expression of foreign proteins under the control of thelac promoter. Journal of Industrial Microbiology 16, 145–154 (1996). https://doi.org/10.1007/BF01569997

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