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Interaction of Bovine Lymphocytes with Products of Shiga Toxin-Producing Escherichia coli

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Shiga Toxin-Producing E. coli

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2291))

Abstract

Shiga toxin-producing Escherichia coli (STEC) produce a number of virulence factors that interfere with lymphocyte functions, including mitogen- and antigen-activated proliferation and pro-inflammatory cytokine synthesis. Here we describe how to isolate lymphocyte subsets from bovine peripheral blood as well as methods that we have used to study the effects of STEC products on lymphocyte proliferation and cytokine production. We also describe an assay that allows for the detection of association of a given protein with lymphocytes.

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Acknowledgments

This work was funded by a Principal’s Career Development Ph.D. Scholarship from the Roslin Institute and the University of Edinburgh.

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Authors and Affiliations

  1. The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK

    Andrew G. Bease, Robin L. Cassady-Cain & Mark P. Stevens

Authors
  1. Andrew G. Bease
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  2. Robin L. Cassady-Cain
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  3. Mark P. Stevens
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Corresponding author

Correspondence to Andrew G. Bease .

Editor information

Editors and Affiliations

  1. University of East Anglia, Norwich Medical School, Norwich, UK

    Stephanie Schüller

  2. National Reference Laboratory for E. coli and Shigellae, National Institute of Public Health, Prague, Czech Republic

    Martina Bielaszewska

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Bease, A.G., Cassady-Cain, R.L., Stevens, M.P. (2021). Interaction of Bovine Lymphocytes with Products of Shiga Toxin-Producing Escherichia coli. In: Schüller, S., Bielaszewska, M. (eds) Shiga Toxin-Producing E. coli . Methods in Molecular Biology, vol 2291. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1339-9_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1339-9_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1338-2

  • Online ISBN: 978-1-0716-1339-9

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