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Detection of Insertions/Deletions Within SIRT1, SIRT2 and SIRT3 Genes and Their Associations with Body Measurement Traits in Cattle

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Abstract

Growth traits are complex quantitative traits controlled by numerous candidate genes, and they can be well-evaluated using body measurement traits. As the members of the nicotinamide adenine dinucleotide-dependent family of histone deacetylases, class I sirtuin genes (including SIRT1, SIRT2 and SIRT3) play crucial roles in regulating lipid metabolism, cellular growth and metabolism, suggesting that they are potential candidate genes affecting body measurement traits in animals. Hence, the objective of this work aimed to detect novel insertions/deletions (indels) of SIRT1, SIRT2 and SIRT3 genes in 955 cattle belonging to five breeds, as well as to evaluate their effects on body measurement traits. Herein, the novel 12-bp indel of SIRT1 gene, the 7-bp indel of SIRT2 gene and the 26-bp indel of SIRT3 gene were firstly reported, respectively. The association analysis indicated that the indels within SIRT1 and SIRT2 genes were significantly associated with body measurement traits such as body weight, chest circumference, height at hip cross, hip width, body height, etc. (P < 0.05 or P < 0.01). Therefore, based on these findings, the two novel indel variants within bovine SIRT1 and SIRT2 genes could be considered as potential molecular markers for growth traits in cattle selection practices and breeding.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31672400), the program of National Beef Cattle and Yak Industrial Technology System (No. CARS-37) and the National Project of Scientific Innovation Experiment for Undergraduate of Northwest A&F University (201710712013).

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Authors and Affiliations

  1. Shaanxi Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Yunyun Jin, Qing Yang, Jiayang Gao, Qi Tang, Bo Duan, Ting Yu, Ruihua Dang, Chuzhao Lei, Hong Chen & Xianyong Lan

  2. Xianan Cattle Technology Development Company, Biyang, 463700, Henan, People’s Republic of China

    Xinglei Qi

  3. Bureau of Animal Husbandry, Biyang, 463700, Henan, People’s Republic of China

    Xinglei Qi

  4. Animal Husbandry Technology Promotion Station of Jiangxi, Nanchang, 330000, Jiangxi, People’s Republic of China

    Jiming Liu & Rongmin Wang

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  1. Yunyun Jin
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  2. Qing Yang
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  11. Chuzhao Lei
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Correspondence to Xianyong Lan.

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Jin, Y., Yang, Q., Gao, J. et al. Detection of Insertions/Deletions Within SIRT1, SIRT2 and SIRT3 Genes and Their Associations with Body Measurement Traits in Cattle. Biochem Genet 56, 663–676 (2018). https://doi.org/10.1007/s10528-018-9868-3

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