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Two novel polymorphisms of bovine SIRT2 gene are associated with higher body weight in Nanyang cattle

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Abstract

Identification of polymorphisms associated with economic traits is important for successful marker-assisted selection in cattle breeding. The family of mammalian sirtuin regulates many biological functions, such as life span extension and energy metabolism. SIRT2, a most abundant sirtuin in adipocytes, acts as a crucial regulator of adipogenic differentiation and plays a key role in controlling adipose tissue function and mass. Here we investigated single nucleotide polymorphisms (SNPs) of bovine SIRT2 in 1226 cattle from five breeds and further evaluated the effects of identified SNPs on economically important traits of Nanyang cattle. Our results revealed four novel SNPs in bovine SIRT2, one was located in intronic region and the other three were synonymous mutations. Linkage disequilibrium and haplotype analyses based on the identified SNPs showed obvious difference between crossbred breed and the other four beef breeds. Association analyses demonstrated that SNPs g.17333C > T and g.17578A > G have a significantly effect on 18-months-old body weight of Nanyang population. Animals with combined genotype TTGG at the above two loci exhibited especially higher body weight. Our data for the first time demonstrated that polymorphisms in bovine SIRT2 are associated with economic traits of Nanyang cattle, which will be helpful for future cattle selection practices.

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Acknowledgments

This work was supported by the National 863 Program of China (Grant No. 2013AA102505), National Natural Science Foundation of China (No. 31272408), Program of National Beef Cattle Industrial Technology System (No. CARS-38) and Agricultural Science and Technology Innovation Projects of Shaanxi Province (No. 2012NKC01-13).

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Authors and Affiliations

  1. Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, No.22 Xinong Road, Yangling, 712100, Shaanxi, China

    Xiaomei Sun, Mingxun Li, Dan Hao, Liushuai Hua, Xianyong Lan, Chuzhao Lei, Shenrong Hu & Hong Chen

  2. Xianan Cattle Technology & Development Company, Biyang, Henan, 463700, China

    Xinglei Qi

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  1. Xiaomei Sun
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  2. Mingxun Li
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  3. Dan Hao
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  4. Liushuai Hua
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  5. Xianyong Lan
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  6. Chuzhao Lei
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  7. Shenrong Hu
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  8. Xinglei Qi
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  9. Hong Chen
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Correspondence to Hong Chen.

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Sun, X., Li, M., Hao, D. et al. Two novel polymorphisms of bovine SIRT2 gene are associated with higher body weight in Nanyang cattle. Mol Biol Rep 42, 729–736 (2015). https://doi.org/10.1007/s11033-014-3821-2

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  • DOI: https://doi.org/10.1007/s11033-014-3821-2

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