Abstract
Morphological and molecular characteristics were studied in six wild species of Passiflora. There were statistically significant differences among these six species for all characteristics studied. Intra-specific variability was observed for number of flowers, number of fruits, number of seeds, fruit length, fruit width and leaf area. Cluster analysis using morphological data showed three groups: 1) P. palmeri var. sublanceolata, P. morifolia and P. foetida var. foetida, 2) P. coriacea and P. micropetala, and 3) P. suberosa. The dendrogram constructed using randomly amplified polymorphic DNA (RAPD) data showed six different groups for each species. The genetic distances among the 24 accessions ranged from 0.05 (between P. morifolia accessions P1 and P3) to 0.95 (P. coriacea accession 31 and P. palmeri var. sublanceolata accession 49). The species showed high morphological and molecular inter- and intraspecific variability.
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Abbreviations
- RAPD:
-
randomly amplified polymorphic DNA
- UPGMA:
-
unweighted pair-group method with arithmetic means
References
Aukar, A.P.A., Lemos, E.G.M., Oliveira, J.C.: Genetic variations among passion fruit species using RAPD markers. — Rev. Bras. Fruticult. 24: 738–740, 2002.
Bellon, G., Faleiro, F.G., Junqueira, K.P., Junqueira, N.T.V., Santos, E.C., Braga, M.F., Guimaraes, C.T.: Genetic variability of access wild and trade of Passiflora edulis Sims. based on RAPD markers. — Rev. Bras. Fruticult. 29: 124–127, 2007.
Cassiano, A.P.A.A., Lemos, E.G.M., Oliveira, J.C.: Assessment of species of Passiflora through molecular markers RAPD. — Genet. mol. Biol. 21: 214–215, 1998.
Cerqueira-Silva, C.B.M., Cardoso-Silva, C.B., Nonato, J.V., Corrêa, R.X., Oliveira, A.C.: Genetic dissimilarity of ‘yellow’ and ’sleep’ passion fruit accessions based on the fruits physical-chemical characteristics. — Crop Breed. appl. Biotechnol. 9: 210–218, 2009.
Cervi, A.C.: [Passiflora L. (Passifloraceae) species published and described in the last 55 years (1950–2005) in South America.] — Estud. Biol. 27(61): 19–24, 2005. [In Port.]
Crochemore, M.L., Molinari, H.B., Stenzel, N.M.C.: Agromorphological characterization of passion fruit (Passiflora spp.). — Rev. Bras. Fruticult. 25: 5–10, 2003a.
Crochemore, M.L., Molinari, H.B., Vieira, L.G.E.: Genetic diversity in passion fruit (Passiflora spp.) evaluated by RAPD markers. — Braz. Arch. Biol. Technol. 46: 521–527, 2003b.
Doyle, J.J., Doyle, J.L.: Isolation of plant DNA from fresh tissue. — Focus 12: 13–15, 1990.
Fajardo, D., Angel, F., Grum, M., Tohme, J., Lobo, M., Roca, W.M., Sanchez, I.: Genetic variation analysis of the genus Passiflora L. using RAPD markers. — Euphytica 101: 341–347, 1998.
Figueiredo, R.W., Séssa, M. C. M., Holanda, L.F.F., Maia, G.A., Oliveira, G.S.F.: Study of the physical characteristics and performance of yellow passion fruit. — Rev. Bras. Fruticult. 2: 613–617, 1988.
Jordán-Pla, A., Estrelles, E., Boscaciu, M., Soriano, P., Vicente, O., Mateu-Andrés, I.: Genetic variability in the endemic Leucojum valentinum. — Biol. Plant. 53: 317–320, 2009.
Martins, M.R., Oliveira, J.C., Di Mauro, A.O., Silva, P.C.: Assessment of populations of sweet passion fruit (Passiflora alata Curtis) obtained from open pollination. — Rev. Bras. Fruticult. 25: 111–114, 2003.
Primot, S., D’Eeckenbrugge, C.G., Rioux, V., Pérez, J.A.O., Garcin, F.: Morphological variation of three species of passion fruit (Passiflora tripartita var. mollissima, P. tarminiana and P. mixta) and hybrids in Valle del Cauca (Colombia). — Rev. Bras. Fruticult. 27: 467–471, 2005. [In Span.]
Segura, S., D’Eeckenbrugge, G.C., Bohorquez, A., Ollitrault, P., Tohme, J.: An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia. — Genet. Resour. Crop. Evol. 49: 1–10, 2002.
Viana, A.P., Pereira, T.N.S., Pereira, M.G., Souza, M.M., Maldonado, J.F.M., Amaral, A.T., Jr.: Genetic diversity among yellow passion fruit commercial genotypes and among passiflora species using RAPD. — Rev. Bras. Fruticult. 25: 489–493, 2003.
Vieira, M.L.C., Oliveira, C.A., Mayeda, L.Y., Dornelas, M.C., Fungaro, M.H.P.: Study of karyotype and genetic variability found by RAPD in species of passion fluit (Passiflora L.). — Braz. J. Genet. 20(Suppl.): 88, 1997.
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V.: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. — Nucl. Acid Res. 18: 6531–6535, 1990.
Acknowledgements
The authors thank FAPESB for funding the research and for the fellowship to the first author, and IAC (SP), EMBRAPA Cerrados (DF), EMBRAPA-CNPFM (BA) and UENF (RJ) for donating the seeds used in the experiment.
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Viana, A.J.C., Souza, M.M., Araújo, I.S. et al. Genetic diversity in Passiflora species determined by morphological and molecular characteristics. Biol Plant 54, 535–538 (2010). https://doi.org/10.1007/s10535-010-0094-z
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DOI: https://doi.org/10.1007/s10535-010-0094-z