Abstract
Fifty-six sequences encoding the pina protein were characterized from three species or subspecies of einkorn wheat. These sequences contained 1,595 nucleotides, including 1,270 conserved sites, 21 single nucleotide polymorphisms (SNPs), and 16 indels. The average frequency of SNPs and indels was one out of 76.1 and 99.9 bases, respectively. Five SNPs and no indels were found in the translated sequences. Fourteen haplotypes were defined, and the accessions in each haplotype ranged from 1 to 18. There were nine haplotypes in Triticum monococcum ssp. aegilopoides, eight in T. monococcum ssp. monococcum, and two in T. urartu. Phylogenetic analysis showed that pina genes from different species or subspecies could be clearly differentiated based on the open reading frame. Genes from T. urartu grouped together, whereas genes from T. monococcum ssp. aegilopoides and T. monococcum ssp. monococcum were shared by three and two clusters, respectively. Both the haplotype and phylogenetic analyses indicated that T. monococcum ssp. aegilopoides was more diverse. These results would contribute to the understanding of functional aspects and efficient utilization of pina genes.
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Acknowledgments
All the PI marked materials were kindly supplied by National Small Grains Collection, U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), USA. This study was supported by the National High Technology Research and Development Program of China (863 program 2006AA10Z179 and 2006AA10Z1F8), the Key Technologies R&D Program of China (2006BAD01A02-23 and 2006BAD13B02), and the FANEDD project (200357 and 200458) from the Ministry of Education, China. Dr. Y.-M. Wei was supported by the Program for New Century Excellent Talents in Universities of China (NCET-05-0814). Prof. Y.-L. Zheng was supported by the Program for Changjiang Scholars and Innovative Research Teams in Universities of China (IRT0453).
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Qing Chen and Peng-Fei Qi contributed equally to this article.
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Chen, Q., Qi, PF., Wei, YM. et al. Molecular Characterization of the pina Gene in Einkorn Wheat. Biochem Genet 47, 384–396 (2009). https://doi.org/10.1007/s10528-009-9239-1
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DOI: https://doi.org/10.1007/s10528-009-9239-1