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A mild cold stress that increases resistance to heat lowers FOXO translocation in Drosophila melanogaster

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Abstract

Previous studies have shown that subjecting Drosophila melanogaster flies to a mild stress at young or middle age can increase lifespan and resistance to severe stresses throughout life and that the NF-κB-like transcription factor DIF, the 70 kDa heat-shock proteins, and the Drosophila Forkhead box class O (dFOXO) transcription factor could explain some of these effects. The present study showed that two dFOXO mutants do not survive longer heat if previously subjected to a mild cold stress, contrarily to wild-type flies. This cold pretreatment had nearly no effect on dFOXO nuclear translocation in wild-type males. Heat stress strongly increased dFOXO translocation, but this effect was lowered in cold-pretreated males. Because cold-pretreated wild-type males survived longer heat and had nevertheless a lower dFOXO translocation after this heat stress, one can conclude that dFOXO is required to resist heat but that the cold pretreatment makes that other mechanisms partly substitute to dFOXO translocation.

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Acknowledgements

The dFOXO 21 and dFOXO 25 flies were kindly provided by Hugo Stocker and Igor Vuillez (Institute of Molecular Systems Biology, ETH, Zurich, Switzerland) and the dFOXO Δ94 flies by Julien Colombani (Institut of Biology Valrose, University of Nice-Sophia-Antipoplis, Nice, France). The primary anti-Foxo antibody is a kind gift of Pierre Léopold (Institut of Biology Valrose, University of Nice-Sophia-Antipoplis, Nice, France).

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Authors and Affiliations

  1. Centre de Biologie du Développement (CBD), Centre de Biologie Intégrative (CBI Toulouse), Université de Toulouse, CNRS, UPS, Toulouse, France

    Cédric Polesello

  2. Centre de Recherches sur la Cognition Animale (CRCA), Centre de Biologie Intégrative (CBI Toulouse), Université de Toulouse, CNRS, UPS, Toulouse, France

    Eric Le Bourg

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  1. Cédric Polesello
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  2. Eric Le Bourg
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Correspondence to Eric Le Bourg.

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Polesello, C., Le Bourg, E. A mild cold stress that increases resistance to heat lowers FOXO translocation in Drosophila melanogaster . Biogerontology 18, 791–801 (2017). https://doi.org/10.1007/s10522-017-9722-8

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