Abstract
A cold pretreatment has various positive effects on aging, longevity, and resistance to some stresses in Drosophila melanogaster flies. Removing live yeast from food has either favorable or deleterious effects on these traits. This study combined a cold pretreatment and removal of live yeast in the same flies, in order to know whether higher favorable effects would be observed with this combination than with each treatment alone. Each treatment had positive effects on climbing activity and their combination had additive effects, thus giving the highest climbing scores in flies subjected to the two treatments and the lowest ones in flies with live yeast and no cold pretreatment. Therefore, combining the two treatments had favorable effects if each of them had favorable effects. When removing live yeast had very deleterious effects, as on resistance to cold, a cold pretreatment was unable to reverse these effects, which was not unexpected. Finally, when removal had no effect or not a tragic negative effect, as on resistance to infection or to heat, a cold pretreatment was neutral or slightly compensated the negative effects of live yeast removal. These results lead to the conclusion that next studies using a combination of two mild stresses having beneficial effects on aging and longevity could bring more positive effects than each mild stress alone.
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Abete P, Testa G, Galizia G, Mazzella F, Della Morte D, De Santis D, Calabrese C, Cacciatore F, Gargiulo G, Ferrara N, Rengo G, Sica V, Napoli C, Rengo F (2005) Tandem action of exercise training and food restriction completely preserves ischemic preconditioning in the aging heart. Exp Gerontol 40:43–50
Alpatov WW (1930) Experimental studies on the duration of life. XIII. The influence of different feeding during the larval and imaginal stages of life of the imago of Drosophila melanogaster. Am Nat 64:37–55
Calabrese EJ (2008) What is hormesis? In: Le Bourg E, Rattan SIS (eds) Mild stress and healthy aging. Applying hormesis in aging research and interventions. Springer, Berlin, pp 1–19
Carey JR, Liedo P, Harshman L, Zhang Y, Müller HG, Partridge L, Wang JL (2002) Life history response of Mediterranean fruit flies to dietary restriction. Aging Cell 1:140–148
Chapman T, Partridge L (1996) Female fitness in Drosophila melanogaster: an interaction between the effect of nutrition and of encounter rate of males. Proc R Soc Lond B 263:755–759
Chippindale AK, Leroi AM, Kim SB, Rose MR (1993) Phenotypic plasticity and selection in Drosophila life-history evolution. I. Nutrition and the cost of reproduction. J Evol Biol 6:171–193
Cooper TM, Mockett RJ, Sohal BH, Sohal RS, Orr WC (2004) Effect of caloric restriction of the housefly, Musca domestica. FASEB J 18:1591–1593
David JR, Van Herrewege J, Fouillet P (1971) Quantitative under-feeding of Drosophila: effects on adult longevity and fecundity. Exp Gerontol 6:249–257
Feany MB, Bender WW (2000) A Drosophila model of Parkinson’s disease. Nature 404:394–398
Forster MJ, Morris P, Sohal RS (2003) Genotype and age influence the effect of caloric intake on mortality in mice. FASEB J. doi:10.1096/fj.02-0533fje
Kopec S (1928) On the influence of intermittent starvation on the longevity of the imaginal stage of Drosophila melanogaster. Br J Exp Biol 5:204–211
Le Bourg E (2007) Hormetic effects of repeated exposures to cold at young age on longevity, aging and resistance to heat or cold shocks in Drosophila melanogaster. Biogerontology 8:431–444
Le Bourg E (2009) Hormesis, aging and longevity. Biochim Biophys Acta 1790:1030–1039
Le Bourg E, Médioni J (1991) Food restriction and longevity in Drosophila melanogaster. Age Nutr 2:90–94
Le Bourg E, Minois N (1996) Failure to confirm increased longevity in Drosophila melanogaster flies submitted to a food restriction procedure. J Gerontol Biol Sci 51A:B280–B283
Le Bourg E, Minois N (1999) A mild stress, hypergravity exposure, postpones behavioral aging in Drosophila melanogaster. Exp Gerontol 34:157–172
Le Bourg E, Minois N, Bullens P, Baret P (2000) A mild stress due to hypergravity exposure at young age increases longevity in Drosophila melanogaster males. Biogerontology 1:145–155
Le Bourg E, Valenti P, Lucchetta P, Payre F (2001) Effects of mild heat shocks at young age on aging and longevity in Drosophila melanogaster. Biogerontology 2:155–164
Le Bourg E, Toffin E, Massé A (2004) Male Drosophila melanogaster flies exposed to hypergravity at young age are protected against a non-lethal heat shock at middle age but not against behavioral impairments due to this shock. Biogerontology 5:431–443
Le Bourg E, Massou I, Gobert V (2009) Cold stress increases resistance to fungal infection throughout life in Drosophila melanogaster. Biogerontology 10:613–625
Le Rohellec M, Le Bourg E (2009) Contrasted effects of suppressing live yeast from food on longevity, aging and resistance to several stresses in Drosophila melanogaster. Exp Gerontol (in press)
Lemaître B, Reichardt JM, Hoffmann JA (1997) Drosophila host defense: differential induction of antimicrobial peptide genes after infection by various classes of microorganisms. Proc Natl Acad Sci USA 94:14614–14619
Miquel J, Lundgren PR, Binnard R (1972) Negative geotaxis and mating behavior in control and gamma-irradiated Drosophila. Drosoph Inf Serv 60:48
Pearl R, Allen L, Penniman WBD (1926) Culture media for Drosophila. II. A new synthesis medium and its influence on fertility at different densities of populations. Am Nat 60:357–366
Rajamohan A, Sinclair BJ (2008) Short-term hardening effects on survival of acute and chronic cold exposure by Drosophila melanogaster larvae. J Insect Physiol 54:708–718
Soh JW, Hotic S, Arking R (2008) Dietary restriction in Drosophila is dependent on mitochondrial efficiency and constrained by preexisting extended longevity. Mech Ageing Dev 128:581–593
Sørensen JG, Sarup P, Kristensen TN, Loeschcke V (2008) Temperature-induced hormesis in Drosophila. In: Le Bourg E, Rattan SIS (eds) Mild stress and healthy aging. Applying hormesis in aging research and interventions. Springer, Berlin, pp 81–96
Sørensen JG, Holmstrup M, Sarup, Loeschcke V (2009) Evolutionary theory and studies of model organisms predict a cautiously positive perspective on the therapeutic use of hormesis for healthy aging in humans. Dose Response (in press)
Tatar M (2007) Diet restriction in Drosophila melanogaster. In: Mobbs CV, Yen K, Hof PR (eds) Mechanisms of caloric restriction in aging. Interdicsipl Top Gerontol. Karger, Basel, pp 115–136
Vaiserman AM, Koshel NM, Litoshenko AY, Mozzhukina TG, Voitenko VP (2003) Effects of X-irradiation in early ontogenesis on the longevity and amount of the S1 nuclease-sensitive DNA sites in adult Drosophila melanogaster. Biogerontology 4:9–14
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Many thanks are due to Vanessa Gobert and Isabelle Massou who were very helpful for performing the infection experiment.
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Le Bourg, É. Combined effects of suppressing live yeast and of a cold pretreatment on longevity, aging and resistance to several stresses in Drosophila melanogaster . Biogerontology 11, 245–254 (2010). https://doi.org/10.1007/s10522-009-9250-2
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DOI: https://doi.org/10.1007/s10522-009-9250-2