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Fasting increases survival to cold in FOXO, DIF, autophagy mutants and in other genotypes of Drosophila melanogaster

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Abstract

Fasting increases survival to a severe cold stress in young and middle-aged wild-type flies, this effect being lowered or absent at old age. As an attempt to determine the mechanisms of this effect, genes involved in metabolism (dFOXO), autophagy (Atg7), innate immunity (Dif 1), and resistance to cold (Frost) were studied. The 12 mutant, RNAi and control lines tested in this study displayed an increased survival to cold after fasting. This shows that fasting has a robust effect on survival to cold in many genotypes, but the mechanism of this effect remains unknown. This mechanism does not seem to be linked to metabolic pathways often considered to play a critical role in ageing and longevity determinations (insulin/insulin-like growth factor-1 pathway and autophagy).

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Acknowledgments

dFOXO, da-GAL4, and Atg flies were kindly provided, respectively, by Cédric Polisello (UMR CNRS 5547, University Paul-Sabatier, Toulouse, France), Guillaume Isabel (UMR CNRS 5169, University Paul-Sabatier, Toulouse, France), and Nadège Minois (Biomedical Sciences Research Complex, University of St Andrews, Scotland). Guillaume Isabel and Hervé Colinet, (UMR CNRS 6553, University Rennes-1, France) were also very helpful in various matters.

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Authors and Affiliations

  1. Centre de Recherches sur la Cognition Animale, UMR CNRS 5169, Université Paul Sabatier, 31062, Toulouse Cedex 9, France

    Éric Le Bourg & Isabelle Massou

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  1. Éric Le Bourg
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  2. Isabelle Massou
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Correspondence to Éric Le Bourg.

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Le Bourg, É., Massou, I. Fasting increases survival to cold in FOXO, DIF, autophagy mutants and in other genotypes of Drosophila melanogaster . Biogerontology 16, 411–421 (2015). https://doi.org/10.1007/s10522-015-9557-0

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  • DOI: https://doi.org/10.1007/s10522-015-9557-0

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