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Context- and dose-dependent modulatory effects of naringenin on survival and development of Drosophila melanogaster

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Abstract

Naringenin, the predominant bioflavonoid found in grapefruit and tomato has diverse bioactive properties that encompass anti-carcinogenic, anti-inflammatory, anti-atherogenic, anti-estrogenic, anti-hyperlipidemic and anti-hyperglycemic characteristics. Naringenin has not been explored for its pro-longevity traits in fruit flies. Therefore, the current study explores its influence on longevity, fecundity, feeding rate, larval development, resistance to starvation stress and body weight in male and female wild-type Drosophila melanogaster Canton-S flies. Flies were fed with normal and high fat diets respectively. The results implied hormetic effects of naringenin on longevity and development in flies. In flies fed with standard and high fat diets, lower concentrations of naringenin (200 and 400 µM) augmented mean lifespan while higher concentrations (600 and 800 µM) were consistently lethal. However, enhanced longevity seen at 400 µM of naringenin was at the expense of reduced fecundity and food intake in flies. Larvae reared on standard diet having 200 µM of naringenin exhibited elevated pupation and emergence as flies. Eclosion time was hastened in larvae reared on standard diet having 200 µM of naringenin. Female flies fed with a standard diet having 200 and 400 µM of naringenin were more resistant to starvation stress. Reduction in body weight was observed in male and female flies fed with a high fat diet supplemented with 200 and 400 µM of naringenin respectively. Collectively, the results elucidated a context- and dose-dependent hormetic efficacy of naringenin that varied with gender, diet and stage of lifecycle in flies.

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Acknowledgments

We thank Dr. N.B. Ramachandra at Mysore University, National Drosophila Stock Centre, India, for providing the wild-type Canton-S fly strain.

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Authors and Affiliations

  1. 206, Structural Biology Lab, Centre for Biomedical Research, VIT University, Vellore, Tamil Nadu, India

    Debarati Chattopadhyay, Soumadeep Sen, Rishita Chatterjee, Debasish Roy, Joel James & Kavitha Thirumurugan

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  1. Debarati Chattopadhyay
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  2. Soumadeep Sen
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  3. Rishita Chatterjee
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  4. Debasish Roy
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  5. Joel James
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  6. Kavitha Thirumurugan
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Correspondence to Kavitha Thirumurugan.

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Chattopadhyay, D., Sen, S., Chatterjee, R. et al. Context- and dose-dependent modulatory effects of naringenin on survival and development of Drosophila melanogaster . Biogerontology 17, 383–393 (2016). https://doi.org/10.1007/s10522-015-9624-6

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