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Culturing of Mouse Mesenchymal Stem Cells on Poly-3-Hydroxybutyrate Scaffolds

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We studied the possibility of long-term culturing of mouse mesenchymal stem cells on a porous scaffold made of biocompatible polymer poly-3-hydroxybutyrate. The cells remained viable for at least 2 months and passed more than 65 population doublings in culture. Culturing on the scaffold did not change surface phenotype of cells. 3D poly-3-hydroxybutyrate scaffolds are appropriate substrate for long-term culturing of mesenchymal stem cells.

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Authors and Affiliations

  1. V. A. Engelhard Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia

    N. V. Andreeva & A. V. Belyavskii

  2. Department of Bioengineering, Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia

    N. V. Andreeva, A. P. Bonartsev, I. I. Zharkova, V. V. Voinova & K. V. Shaitan

  3. Federal Research Centre Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, Russia

    A. P. Bonartsev, T. K. Makhina, V. L. Myshkina & V. V. Voinova

  4. Physical Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia

    E. P. Kharitonova & G. A. Bonartseva

Authors
  1. N. V. Andreeva
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  2. A. P. Bonartsev
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  3. I. I. Zharkova
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  4. T. K. Makhina
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  5. V. L. Myshkina
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  6. E. P. Kharitonova
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  7. V. V. Voinova
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  8. G. A. Bonartseva
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  9. K. V. Shaitan
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  10. A. V. Belyavskii
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Corresponding author

Correspondence to A. P. Bonartsev.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 114–119, April, 2015

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Andreeva, N.V., Bonartsev, A.P., Zharkova, I.I. et al. Culturing of Mouse Mesenchymal Stem Cells on Poly-3-Hydroxybutyrate Scaffolds. Bull Exp Biol Med 159, 567–571 (2015). https://doi.org/10.1007/s10517-015-3015-5

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  • DOI: https://doi.org/10.1007/s10517-015-3015-5

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