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Effect of Factors Produced by the Placenta on Cytokine Secretion by THP-1 Cells Cultured on a 3D Scaffold

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We compared the effects of soluble products from the placenta obtained from women with normal pregnancy and pregnancy complicated by preeclampsia on cytokine secretion by THP-1 cells cultured on a 3D Matrigel scaffold. In the presence of soluble products from all placentas, the cells actively secreted IL-8, MCP-1, and soluble forms of CD14, TNFRI, and TNFRII receptors. Secretion of VEGF was below the spontaneous level. Secretion of IL-6 by THP-1 cells after incubation with soluble products of the placentas obtained during weeks 9-11 of physiological pregnancy and 38-39 of pregnancy complicated by preeclampsia surpassed the spontaneous level. In the presence of soluble factors of trimester I placentas, secretion of IL-6 and soluble form of TNFRI receptor was higher than in the presence of trimester III placental factors. Secretion of IL-6 by THP-1 cells was higher, while secretion of soluble TNFRII receptor was lower in the presence of placentas from women with preeclampsia in comparison with physiological pregnancy.

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Authors and Affiliations

  1. D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia

    Ya. S. Onokhina, T. Yu. L’vova, D. Z. Tsitskarava, D. A. Koren’kov, S. A. Sel’kov & D. I. Sokolov

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  1. Ya. S. Onokhina
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  2. T. Yu. L’vova
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  3. D. Z. Tsitskarava
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  4. D. A. Koren’kov
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  5. S. A. Sel’kov
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  6. D. I. Sokolov
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Corresponding author

Correspondence to D. I. Sokolov.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 217-222, October, 2013

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Onokhina, Y.S., L’vova, T.Y., Tsitskarava, D.Z. et al. Effect of Factors Produced by the Placenta on Cytokine Secretion by THP-1 Cells Cultured on a 3D Scaffold. Bull Exp Biol Med 156, 566–570 (2014). https://doi.org/10.1007/s10517-014-2397-0

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  • DOI: https://doi.org/10.1007/s10517-014-2397-0

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