We studied the effects of secretory products of the placenta obtained from women with normal pregnancy and preeclampsia on the expression of surface markers by THP-1 cells cultured on a 3D Matrigel scaffold. Secretory products of third trimester placentas obtained from women with normal pregnancy reduced the relative number of THP-1 cells expressing CD54 and CD14 molecules and expression of CD14 and CD95 molecules by THP-1 cells in comparison with the effect of secretory products first trimester placentas. In parallel, the intensity of CD49d expression by THP-1 cells increased in the presence of secretory products of third trimester placentas in comparison with the first trimester. No differences in the expression of the studied molecules by THP-1 cells under the effect of placentas from women with physiological pregnancy and patients with preeclampsia were found.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 26-32, January, 2016
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Lvova, T.Y., Stepanova, O.I., Viazmina, L.P. et al. Effect of Factors Secreted by the Placenta on Phenotype of THP-1 Cells Cultured on a 3D Scaffold. Bull Exp Biol Med 161, 162–167 (2016). https://doi.org/10.1007/s10517-016-3368-4
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DOI: https://doi.org/10.1007/s10517-016-3368-4