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Microbiome analysis of the saliva and midgut from partially or fully engorged female adult Dermacentor silvarum ticks in China

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Abstract

Dermacentor silvarum is widely distributed in northern China and transmits several pathogens that cause diseases in humans and domestic animals. We analysed the comprehensive bacterial community of the saliva and midgut from partially and fully engorged female adult D. silvarum. Dermacentor silvarum samples were collected from Guyuan, China. Bacterial DNA was extracted from the saliva and midgut contents of partially or fully engorged female adult D. silvarum. Sequencing of the V3–V4 hypervariable regions of the 16S rRNA genes was performed using the IonS5TMXL platform. The bacterial diversity in saliva was higher than in the midgut. The bacterial diversity of saliva from fully engorged ticks was greater than in partially engorged tick saliva. The bacterial diversity in midguts from partially engorged ticks was greater than in fully engorged tick midguts. Proteobacteria was the most dominant bacterial phylum in all of the samples. Twenty-nine bacterial genera were detected in all of the samples. Rickettsia, Anaplasma, and Stenotrophomonas were the main genera. The symbionts Coxiella, Arsenophonus, and Wolbachia were also detected in all of the samples. Eight bacterial species were identified in all of the experimental samples. Anaplasma marginale was reported for the first time in D. silvarum.

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Funding

This research was financially supported by a grant from the National Science Foundation for Young Scientists of China (No. 31902294), the Youth Science Foundation of Hunan Agricultural University (No. 18QN24), the Natural Science Foundation of Hunan Province, China (No. 2018JJ2167), and the Double First-Class Construction Project of Hunan Agricultural University (No. SYL201802016).

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  1. College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan Province, China

    De-Yong Duan, Guo-Hua Liu & Tian-Yin Cheng

  2. Laboratory of Molecular Physiology, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan Province, China

    Tian-Yin Cheng

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Duan, DY., Liu, GH. & Cheng, TY. Microbiome analysis of the saliva and midgut from partially or fully engorged female adult Dermacentor silvarum ticks in China. Exp Appl Acarol 80, 543–558 (2020). https://doi.org/10.1007/s10493-020-00478-2

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