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Exploitation of new endophytic bacteria and their ability to promote sugarcane growth and nitrogen nutrition

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Abstract

Few studies have evaluated endophytic bacteria in relation to plant growth promotion, nitrogen uptake and biological control. The aim of this study was to molecularly and physiologically characterize thirteen endophytic bacteria strains, evaluate their biological control properties and their ability to promote plant growth and plant N nutrition. All the strains produced indole acetic acid and promoted increase of plant biomass, N accumulative amount and N-use efficiency index. None of the strains carries the nifH gene. Four strains stimulated plant nitrate reductase activity, four solubilized phosphate, nine produced siderophores and none produced HCN. Seven strains inhibited Bipolaris sacchari growth and one was antagonistic to Ceratocystis paradoxa. The pathogens were inhibited by the production of diffusible and volatile metabolites by the bacterial strains. Moreover, this is the first study to demonstrate the effect of Delftia acidovorans on sugarcane plant growth, nitrogen metabolism improvement and antagonism to B. sacchari. The most efficient strains in promoting plant growth and exhibiting antagonistic activities towards fungal pathogens were Herbaspirillum frinsingense (IAC-BECa-152) and three Pantoea dispersa strains (IAC-BECa-128, IAC-BECa-129, and IAC-BECa-132). These bacteria show potential to be used as inoculants for sustainable agricultural management, mainly at the seedling production phase.

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Acknowledgements

The authors acknowledge FAPESP financial support and CAPES for Master and Post-doctoral fellowships. This research was supported by BIOEN/FAPESP (2008/56147-1), The Netherlands Organization for Scientific Research (NWO-729.004.013), and the CNPq/NWO Program (Project Number 456420/2013-4).

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Authors and Affiliations

  1. Center of Soil and Environmental Resources, Agronomic Institute/IAC, P.O. Box-28, Campinas, SP, 13010-970, Brazil

    Adriana Parada Dias da Silveira, Raquel de Paula Freitas Iório, Fernanda Castro Correia Marcos, Ana Olívia Fernandes & Matheus Aparecido Pereira Cipriano

  2. Center of Sugarcane, Agronomic Institute/IAC, Ribeirão Prêto, SP, 14001-970, Brazil

    Silvana Aparecida Creste Dias de Souza

  3. Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands

    Eiko Eurya Kuramae

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  1. Adriana Parada Dias da Silveira
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APDS conceived the study. APDS, RPFI, FCCM, AOF, SCS and MAPC performed the experiments. APDS, RPFI, EEK and MAPC wrote the paper.

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Correspondence to Adriana Parada Dias da Silveira.

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da Silveira, A.P.D., Iório, R.P.F., Marcos, F.C.C. et al. Exploitation of new endophytic bacteria and their ability to promote sugarcane growth and nitrogen nutrition. Antonie van Leeuwenhoek 112, 283–295 (2019). https://doi.org/10.1007/s10482-018-1157-y

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