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Diversity of Culturable Plant Growth-Promoting Bacterial Endophytes Associated with Sugarcane Roots and Their Effect of Growth by Co-Inoculation of Diazotrophs and Actinomycetes

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Abstract

Application of environmentally friendly agents to reduce the use of chemicals and to enhance growth of plants is an ultimate goal of sustainable agriculture. The use of plant growth-promoting endophytes has become of great interest as a way to enhance plant growth and additionally protect plants from phytopathogens. In this study, 135 isolates of endophytic bacteria including actinomycetes were isolated from roots of commercial sugarcane plants cultivated in Thailand and were characterized for plant growth-promoting (PGP) traits. Based on morphological and 16S rRNA sequence analysis, the endophytes were distributed into 14 genera of which the most dominant species belong to Bacillus, Enterobacter, Microbispora, and Streptomyces. Two strains of endophytic diazotrophs, Bacillus sp. EN-24 and Enterobacter sp. EN-21; and two strains of actinomycetes, Microbispora sp. GKU 823 and Streptomyces sp. GKU 895, were selected based on their PGP traits including 1-aminocyclopropane-1-decarboxylate deaminase, indole-3-acetic acid, nitrogen fixation, phosphate solubilization, and siderophore production for evaluation of sugarcane growth enhancement by individual and co-inoculation. Sixty days after co-inoculation by endophytic diazotrophs and actinomycetes, the growth parameters of sugarcane plants were significantly greater than that of individual and un-inoculated plants. The results indicated that these endophytes have high potential as PGP agents that could be applied to promote sugarcane growth and could be developed as active added value biofertilizers in the future.

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Acknowledgments

WK was awarded a Ph.D. scholarship by the Royal Golden Jubilee of the Thailand Research Fund (RGJ-TRF). The authors thank the Soil Microbiology Research Group, Department of Agriculture for their support and for providing facilities for ARA assay. This work was supported by Thailand Research Fund under Grant No. BRG5880004; Faculty of Science, Kasetsart University under Grant No. RFG 1-11 and Mitr Phol Sugarcane Research Center.

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  1. Department of Genetics, Faculty of Science, Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok, 10900, Thailand

    Worarat Kruasuwan & Arinthip Thamchaipenet

  2. Center for Advanced Studies in Tropical Natural Resources, NRU–KU, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand

    Arinthip Thamchaipenet

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  1. Worarat Kruasuwan
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Kruasuwan, W., Thamchaipenet, A. Diversity of Culturable Plant Growth-Promoting Bacterial Endophytes Associated with Sugarcane Roots and Their Effect of Growth by Co-Inoculation of Diazotrophs and Actinomycetes. J Plant Growth Regul 35, 1074–1087 (2016). https://doi.org/10.1007/s00344-016-9604-3

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