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Paenibacillus vini sp. nov., isolated from alcohol fermentation pit mud in Sichuan Province, China

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Abstract

A novel facultatively anaerobic bacterial strain, designated LAM0504T, was isolated from a pit mud of Luzhou flavour liquor alcohol fermentation in Sichuan Province, China. Cells of strain LAM0504T were observed to be Gram-stain negative, spore-forming, rod shaped and motile by means of peritrichous flagella. Strain LAM0504T was found to be able to grow at 20–48 °C (optimum: 30 °C), pH 5.0–9.0 (optimum: 7.0) and 0–3 % NaCl (w/v) (optimum: 1.0 %). The 16S rRNA gene sequence similarity analysis showed that strain LAM0504T was most closely related to Paenibacillus konsisdensis JCM 14798T, Fontibacillus phaseoli LMG 27589T and Paenibacillus motobuensis JCM 12774T, with 97.0, 96.8 and 96.7 % sequence similarity, respectively. The DNA–DNA hybridization value between strain LAM0504T and P. konsisdensis JCM 14798T was 53.3 ± 1.2 %. The genomic DNA G+C content of strain LAM0504T was 43.0 mol% as determined by the Tm method. The major fatty acids of strain LAM0504T were identified as anteiso-C15:0, C16:0 and iso-C15:0. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as MK-7. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, two unidentified glycolipids and three unidentified lipids. On the basis of its physiological and phylogenetic characteristics, strain LAM0504T is concluded to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus vini sp. nov. is proposed. The type strain is LAM0504T (=ACCC 06420T = JCM 19842T).

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Acknowledgments

We would like to thank Professor Aharon Oren from The Hebrew University of Jerusalem for assistance with Latin in deriving the specific epithet for strain LAM0504T. We also thank Professor Min Wu from Zhejiang University for his technique assistant on the chemotaxonomic analyses. This work was supported by National Nonprofit Institute Research Grant of CAAS (No. 2014-30), Fund for Innovation team project of environmental pollution preventing and controlling technology (No. 670100656), National Key Technology R&D Program of China (Nos. 2013BAD05B04F02 and 2011BAD11B05), Foundation of the Key Laboratory of Development and Application of Rural Renewable Energy (MOA, China) (No. 2013002), and the Science Foundation of Modern Farming Group (No. MF20100518).

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Authors and Affiliations

  1. Key Laboratory of Microbial Resources Collection and Preservation, Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People’s Republic of China

    Xiao-Rong Chen, Yan-Wei Wang, Hui-Min Wang, De-Long Kong, Xiang Guo, Yi-Qing Zhou & Zhi-Yong Ruan

  2. College of Environmental and Biological Engineering, Chongqing Technology and Business University, Chongqing, 400067, People’s Republic of China

    Xiao-Rong Chen & Cheng-Bin Shao

  3. Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Chengdu, 610041, People’s Republic of China

    Ming-Xiong He

  4. College of Environmental and Chemical Engineering, Dalian University, Dalian, 116622, People’s Republic of China

    Ke-Dong Ma

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  1. Xiao-Rong Chen
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Correspondence to Cheng-Bin Shao or Zhi-Yong Ruan.

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Chen, XR., Shao, CB., Wang, YW. et al. Paenibacillus vini sp. nov., isolated from alcohol fermentation pit mud in Sichuan Province, China. Antonie van Leeuwenhoek 107, 1429–1436 (2015). https://doi.org/10.1007/s10482-015-0438-y

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