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Paenibacillus salinicaeni sp. nov., isolated from saline silt sample

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Abstract

A novel facultatively anaerobic bacterium, designated strain LAM0A28T, was isolated from a saline silt sample collected from the Chinese Sea of Death located in Suining city, Sichuan province, China. Cells of strain LAM0A28T were observed to be Gram-stain positive, motile, endospore-forming and straight-rod shaped. Strain LAM0A28T was found to be able to grow at 15–45 °C (optimum: 30–35 °C), pH 5.0–10.0 (optimum: 7.5) and 0–5 % NaCl (w/v) (optimum: 0.5 %). The 16S rRNA gene sequence similarity analysis showed that strain LAM0A28T is closely related to Paenibacillus jilunlii DSM 23019T (97.5 %) and Paenibacillus graminis DSM 15220T (97.2 %). The DNA–DNA hybridization values between the isolate and P. jilunlii DSM 23019T, P. graminis DSM 15220T were 30.2 ± 1.6 % and 44.7 ± 2.1 %, respectively. The DNA G+C content was found to be 51.2 mol% as determined by the T m method. The major cellular fatty acids were identified as anteiso-C15:0, C16:0, iso-C16:0 and C14:0. The major isoprenoid quinone was identified as MK-7. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids and six unidentified lipids. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, strain LAM0A28T is concluded to represent a novel species within the genus Paenibacillus, for which the name Paenibacillus salinicaeni sp. nov. is proposed. The type strain is LAM0A28T (=ACCC 00741T = JCM 30850T).

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Acknowledgments

We would like to thank Professor Aharon Oren from The Hebrew University of Jerusalem for assistance with Latin in deriving the specific epithet for the strain LAM0A28. This work was supported by Foundation of the Key Laboratory of Development and Application of Rural Renewable Energy (MOA, China) (No. 201502), National Nonprofit Institute Research Grant of CAAS (No. 2014-30), National Key Technology R&D Program of China (No. 2013BAD05B04F02) and National Infrastructure of Microbial Resources.

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Authors and Affiliations

  1. Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biogas Institute of Ministry of Agriculture, Chengdu, 610041, People’s Republic of China

    Xiang Guo, Yan-Wei Wang, Ming-Xiong He & Guo-Quan Hu

  2. Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People’s Republic of China

    Xiang Guo, Shan Zhou, Hui-Min Wang, De-Long Kong, Jie Zhu, Wei-Wei Dong, Bing-Qiang Zhao & Zhi-Yong Ruan

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  1. Xiang Guo
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Correspondence to Guo-Quan Hu or Zhi-Yong Ruan.

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Guo, X., Zhou, S., Wang, YW. et al. Paenibacillus salinicaeni sp. nov., isolated from saline silt sample. Antonie van Leeuwenhoek 109, 721–728 (2016). https://doi.org/10.1007/s10482-016-0674-9

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