Abstract
A Gram-positive, thermophilic, strictly aerobic bacterium, designated WP-1T, was isolated from a sediment sample from a hot spring in Fujian province of China and subjected to a polyphasic taxonomic study. Cells of strain WP-1T were rods (~0.6–0.8 × 2.5–3.5 μm) and motile by means of peritrichous flagella. Endospores were ellipsoidal in terminal or subterminal positions. Strain WP-1T grew at 37–60 °C (optimum 42–45 °C), 0–3 % NaCl (optimum 1 %, w/v) and pH 3.0–9.0 (optimum pH 6.5–7.0). The predominant menaquinone was MK-7. The major fatty acids were anteiso-C15:0, iso-C16:0, C16:0 and anteiso-C17:0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two glycolipids, two unidentified phospholipids and two unknown polar lipids. The cell-wall peptidoglycan contained meso-diaminopimelic acid (meso-DAP). The G + C content of the genomic DNA was 52.5 %. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain WP-1T is a member of the genus Paenibacillus and exhibited sequence similarity of 99.3 % to Paenibacillus macerans DSM 24T and both strains represented a separate lineage from all other Paenibacillus species. However, the level of DNA–DNA relatedness between strain WP-1T and P. macerans DSM 24T was 34.0 ± 4.7 %. On the basis of phylogenetic, physiological and chemotaxonomic analysis data, strain WP-1T is considered to represent as a novel species of the genus Paenibacillus, for which the name Paenibacillus thermophilus sp. nov., is proposed, with the type strain WP-1T (=DSM 24746T = JCM 17693T = CCTCC AB 2011115T).
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Acknowledgments
The authors are grateful to Dr Hans-Peter Klenk of the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) for kindly providing reference type strain P. macerans DSM 24T. This work was supported by the National Natural Science Foundation of China (NSFC, 31000001). W-J Li was also supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences.
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Zhou, Y., Gao, S., Wei, DQ. et al. Paenibacillus thermophilus sp. nov., a novel bacterium isolated from a sediment of hot spring in Fujian province, China. Antonie van Leeuwenhoek 102, 601–609 (2012). https://doi.org/10.1007/s10482-012-9755-6
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DOI: https://doi.org/10.1007/s10482-012-9755-6