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Design and Development of an In Vitro Tear Replenishment System

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Abstract

Understanding the cellular and molecular mechanisms of the corneal tissue and translating them into effective therapies requires organotypic culture systems that can better model the physiological conditions of the front of the eye. Human corneal in vitro models currently exist, however, the lack of tear replenishment limits corneal in vitro models’ ability to accurately simulate the physiological environment of the human cornea. The tear replenishment system (TRS), a micro-fluidic device, was developed to mimic the in vivo tear replenishment in the human eye in an in vitro corneal model. The TRS is capable of generating adjustable intermittent flow from 0.1 µL in every cycle. The TRS is a sterilizable device that is designed to fit standard 6-well cell culture plates. Experiments with the corneal models demonstrated that exposure to the TRS did not damage the integrity of the stratified cell culture. Contact lenses “worn” by the in vitro corneal model also remained moist at all times and the cytotoxicity of BAK could also be verified using this model. These in vitro results confirmed that the TRS presents novel avenues to assess lens-solution biocompatibility and drug delivery systems in a physiologically relevant milieu.

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Acknowledgments

The funding for this project was provided by the Natural Sciences and Engineering Research Council of Canada and CibaVision (now Alcon). Authors also would like to thank Jason Benninger for his technical assistance in the manufacturing of the microfluidics parts and Christopher Amos from CIBA Vision for fruitful discussion related to the development of this in vitro model.

Conflict of interest

Ann M. Wright is an employee of Alcon (formerly CIBA Vision). In the past 4 years, the corresponding author (MG) has received funding from CIBA Vision/Alcon and Johnson & Johnson.

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Authors and Affiliations

  1. Systems Design Engineering, University of Waterloo, 200 University Ave., Waterloo, ON, Canada

    Saman Mohammadi, Cameron Postnikoff & Maud Gorbet

  2. Alcon/CIBAVision, Duluth, GA, USA

    Ann M. Wright

  3. School of Optometry and Vision Science, University of Waterloo, Waterloo, ON, Canada

    Maud Gorbet

Authors
  1. Saman Mohammadi
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  2. Cameron Postnikoff
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  3. Ann M. Wright
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  4. Maud Gorbet
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Correspondence to Maud Gorbet.

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Associate Editor Estefanía Peña oversaw the review of this article.

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Mohammadi, S., Postnikoff, C., Wright, A.M. et al. Design and Development of an In Vitro Tear Replenishment System. Ann Biomed Eng 42, 1923–1931 (2014). https://doi.org/10.1007/s10439-014-1045-1

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  • DOI: https://doi.org/10.1007/s10439-014-1045-1

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