Abstract
This review focuses on applying nanotechnology to foodborne pathogen detection. Because of low infectious doses for most foodborne pathogens, the rapid and sensitive detection methods are essential to ensure the food safety. The advances in the development of nanomaterials have stimulated worldwide research interests in their applications for bioanalysis. The conjugation of biomolecules with nanomaterials is the foundation of nano-biorecognition. A variety of strategies including antibody–antigen, adhesin–receptor, antibiotic, and complementary DNA sequence recognitions have been explored for specific recognition between target bacterial cells and bio-functionalized nanomaterials. The incorporation of these bio-functionalized nanomaterials into current pathogen detection methods has led to rapid and nearly real-time pathogen detection (as short as a few minutes), improved sensitivity (single bacterial cell), and simultaneous detection of multiple micro-organisms from either nutrient broth, liquid or solid food products, or biofilms. The unique properties of nanomaterials in physical strength, chemical reactivity, electrical conductance, magnetism and optical effects make them promising in the development of practical biosensors with emphasis on device portability and simplicity in sample preparation, and the improvement of current pathogen detection methods.
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Yang, H., Li, H. & Jiang, X. Detection of foodborne pathogens using bioconjugated nanomaterials. Microfluid Nanofluid 5, 571–583 (2008). https://doi.org/10.1007/s10404-008-0302-8
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DOI: https://doi.org/10.1007/s10404-008-0302-8