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Expression of β-1,3-glucanase (GLU) and phenylalanine ammonia-lyase (PAL) genes and their enzymes in tomato plants induced after treatment with Bacillus subtilis CBR05 against Xanthomonas campestris pv. vesicatoria

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Abstract

When 4-week-old tomato plants, which developed from seeds treated with antagonist Bacillus subtilis CBR05 before sowing, were challenged with Xanthomonas campestris pv. vesicatoria (XCV), activities of plant-defense enzymes β-1,3-glucanase (GLU, 42.5%) and phenylalanine ammonia-lyase (PAL; 93.9%) were significantly higher than in control plants at 72 h after inoculation with XCV. Also, GLU- and PAL-related genes were upregulated 2- and 3-fold, respectively, in these plants. Our results suggest that expression of GLU- and PAL-related genes in plants from seeds pretreated with B. subtilis are pivotal for inducing disease resistance against XCV.

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Acknowledgements

This paper was written as part of Konkuk University’s research support program for its faculty on sabbatical leave in 2015.

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Authors and Affiliations

  1. Department of Bioresource and Food Science, Konkuk University, Seoul, Republic of Korea

    Murugesan Chandrasekaran, Shimels Tilahun Belachew, Ee Yoon & Se Chul Chun

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  1. Murugesan Chandrasekaran
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  2. Shimels Tilahun Belachew
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  3. Ee Yoon
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  4. Se Chul Chun
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Correspondence to Se Chul Chun.

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Chandrasekaran, M., Belachew, S.T., Yoon, E. et al. Expression of β-1,3-glucanase (GLU) and phenylalanine ammonia-lyase (PAL) genes and their enzymes in tomato plants induced after treatment with Bacillus subtilis CBR05 against Xanthomonas campestris pv. vesicatoria . J Gen Plant Pathol 83, 7–13 (2017). https://doi.org/10.1007/s10327-016-0692-5

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