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Engineering levoglucosan metabolic pathway in Rhodococcus jostii RHA1 for lipid production

  • Metabolic Engineering and Synthetic Biology - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Oleaginous strains of Rhodococcus including R. jostii RHA1 have attracted considerable attention due to their ability to accumulate triacylglycerols (TAGs), robust growth properties and genetic tractability. In this study, a novel metabolic pathway was introduced into R. jostii by heterogenous expression of the well-characterized gene, lgk encoding levoglucosan kinase from Lipomyces starkeyi YZ-215. This enables the recombinant R. jostii RHA1 to produce TAGs from the anhydrous sugar, levoglucosan, which can be generated efficiently as the major molecule from the pyrolysis of cellulose. The recombinant R. jostii RHA1 could grow on levoglucosan as the sole carbon source, and the consumption rate of levoglucosan was determined. Furthermore, expression of one more copy of lgk increased the enzymatic activity of LGK in the recombinant. However, the growth performance of the recombinant bearing two copies of lgk on levoglucosan was not improved. Although expression of lgk in the recombinants was not repressed by the glucose present in the media, glucose in the sugar mixture still affected consumption of levoglucosan. Under nitrogen limiting conditions, lipid produced from levoglucosan by the recombinant bearing lgk was up to 43.54 % of the cell dry weight, which was comparable to the content of lipid accumulated from glucose. This work demonstrated the technical feasibility of producing lipid from levoglucosan, an anhydrosugar derived from the pyrolysis of lignocellulosic materials, by the genetically modified rhodococci strains.

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Acknowledgments

We thank Dr. Lindsay D. Eltis (Department of Microbiology and Immunology, University of British Columbia) for the gift of R. jostii RHA1. We also acknowledge FMIC at WSU for providing us with the technical support and resource of the confocal microscopy.

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Author notes

  1. Jieni Lian

    Present address: Department of Chemical and Biological Engineering, Iowa State University, Ames, IA, 50011, USA

  2. Xiaochen Yu

    Present address: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA, 50011, USA

Authors and Affiliations

  1. Department of Biological Systems Engineering, Washington State University, L. J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA

    Xiaochao Xiong, Jieni Lian, Xiaochen Yu, Manuel Garcia-Perez & Shulin Chen

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  1. Xiaochao Xiong
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Correspondence to Shulin Chen.

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Xiong, X., Lian, J., Yu, X. et al. Engineering levoglucosan metabolic pathway in Rhodococcus jostii RHA1 for lipid production. J Ind Microbiol Biotechnol 43, 1551–1560 (2016). https://doi.org/10.1007/s10295-016-1832-9

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  • DOI: https://doi.org/10.1007/s10295-016-1832-9

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