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Application of enzymatic apple pomace hydrolysate to production of 2,3-butanediol by alkaliphilic Bacillus licheniformis NCIMB 8059

  • Fermentation, Cell Culture and Bioengineering
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

2,3-Butanediol (2,3-BD) synthesis by a nonpathogenic bacterium Bacillus licheniformis NCIMB 8059 from enzymatic hydrolysate of depectinized apple pomace and its blend with glucose was studied. In shake flasks, the maximum diol concentration in fed-batch fermentations was 113 g/L (in 163 h, from the hydrolysate, feedings with glucose) while in batch processes it was around 27 g/L (in 32 h, from the hydrolysate and glucose blend). Fed-batch fermentations in the 0.75 and 30 L fermenters yielded 87.71 g/L 2,3-BD in 160 h, and 72.39 g/L 2,3-BD in 94 h, respectively (from the hydrolysate and glucose blend, feedings with glucose). The hydrolysate of apple pomace, which was for the first time used for microbial 2,3-BD production is not only a source of sugars but also essential minerals.

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Acknowledgments

The presented results were obtained within the scope of the international ERA-IB project (2009–2011) with the acronym PUBB. Financial support was provided by the Polish National Center for Research and Development (NCBiR).

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Authors and Affiliations

  1. Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland

    Aneta M. Białkowska, Ewa Gromek, Joanna Krysiak, Barbara Sikora, Halina Kalinowska, Marzena Jędrzejczak-Krzepkowska, Celina Kubik & Marianna Turkiewicz

  2. Institute of Biochemistry and Biotechnology, Technical University Braunschweig, Spielmannstrasse 7, 38106, Brunswick, Germany

    Siegmund Lang

  3. Thünen Institute of Wood Research, Leuschnerstrasse 91, 21031, Hamburg, Germany

    Fokko Schütt

Authors
  1. Aneta M. Białkowska
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  2. Ewa Gromek
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Correspondence to Halina Kalinowska.

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Białkowska, A.M., Gromek, E., Krysiak, J. et al. Application of enzymatic apple pomace hydrolysate to production of 2,3-butanediol by alkaliphilic Bacillus licheniformis NCIMB 8059. J Ind Microbiol Biotechnol 42, 1609–1621 (2015). https://doi.org/10.1007/s10295-015-1697-3

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