Abstract
2,3-Butanediol (2,3-BD) is a valuable bulk chemical with particular use in industry. 2,3-BD has a potential as solvent and fuel additive, as carrier for pharmaceuticals, or as feedstock for the production of synthetic rubber. Until now, the highest 2,3-BD concentrations were obtained with risk group 2 microorganisms (e.g., Klebsiella oxytoca). In this study, the nonpathogenic bacterium Bacillus licheniformis DSM 8785 was used for 2,3-BD production from glucose. In batch experiments, a maximum 2,3-BD concentration of 72.6 g/L was reached from 180 g/L glucose after 86 h. The yield was 0.42 g/g glucose and the productivity was 0.86 g/(L h). During fed-batch cultivation, 2,3-BD production could be increased up to 144.7 g/L, with a productivity of 1.14 g/(L h). Additionally, repeated batch/fed-batch experiments were conducted using immobilized B. licheniformis in the form of LentiKats®. Results showed a high activity and stability of the immobilizates even after multiple medium replacements, as well as 2,3-BD concentrations, yields, and productivities similar to those obtained with free cells. To our knowledge, these results show the highest 2,3-BD concentration reported so far using a risk group 1 microorganism in general and B. licheniformis in particular. Furthermore, productivity lies in the same range with data reported from risk group 2 strains, which makes B. licheniformis DSM 8785 a suitable candidate for large-scale fermentation processes.
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References
Biswas R, Yamaoka M, Nakayama H, Kondo T, K-i Y, Bisaria V, Kondo A (2012) Enhanced production of 2,3-butanediol by engineered Bacillus subtilis. Appl Microbiol Biotechnol 94:651–658
Celińska E, Grajek W (2009) Biotechnological production of 2,3-butanediol—current state and prospects. Biotechnol Adv 27:715–725
Fulmer EI, Christensen LM, Kendali AR (1933) Production of 2,3-butylene glycol by fermentation. Ind Eng Chem 25:798–800
Garg SK, Jain A (1995) Fermentative production of 2,3-butanediol: a review. Bioresour Technol 51:103–109
Gräfje H, Körnig W, Weitz H-M, Reiß W, Steffan G, Diehl H, Bosche H, Schneider K, Kieczka H (2000) Butanediols, butenediol, and butynediol. In: Bailey JE, Brinker CJ, Cornils B (eds) Ullmann’s encyclopedia of industrial chemistry, 7th edn. Wiley-VCH, Weinheim (electronic release)
Häßler T, Schieder D, Pfaller R, Faulstich M, Sieber V (2012) Enhanced fed-batch fermentation of 2,3-butanediol by Paenibacillus polymyxa DSM 365. Bioresour Technol 124:237–244
Ji X-J, Huang H, Zhu J-G, Ren L-J, Nie Z-K, Du J, Li S (2010) Engineering Klebsiella oxytoca for efficient 2, 3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene. Appl Microbiol Biotechnol 85:1751–1758
Ji X-J, Huang H, Ouyang P-K (2011) Microbial 2,3-butanediol production: a state-of-the-art review. Biotechnol Adv 29:351–364
Jung M-Y, Ng C, Song H, Lee J, Oh M-K (2012) Deletion of lactate dehydrogenase in Enterobacter aerogenes to enhance 2,3-butanediol production. Appl Microbiol Biotechnol 95:461–469
Ma C, Wang A, Qin J, Li L, Ai X, Jiang T, Tang H, Xu P (2009) Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM. Appl Microbiol Biotechnol 82:49–57
Moes J, Griot M, Keller J, Heinzle E, Dunn IJ, Bourne JR (1985) A microbial culture with oxygen-sensitive product distribution as a potential tool for characterizing bioreactor oxygen transport. Biotechnol Bioeng 27:482–489
Nakashimada Y, Kanai K, Nishio N (1998) Optimization of dilution rate, pH and oxygen supply on optical purity of 2,3-butanediol produced by Paenibacillus polymyxa in chemostat culture. Biotechnol Lett 20:1133–1138
Nie Z-K, Ji X-J, Huang H, Du J, Li Z-Y, Qu L, Zhang Q, Ouyang P-K (2011) An effective and simplified fed-batch strategy for improved 2,3-butanediol production by Klebsiella oxytoca. Appl Biochem Biotechnol 163:946–953
Nilegaonkar S, Bhosale SB, Kshirsagar DC, Kapadi AH (1992) Production of 2,3-butanediol from glucose by Bacillus licheniformis. World J Microbiol Biotechnol 8:378–381
Nilegaonkar SS, Bhosale SB, Dandage CN, Kapadi AH (1996) Potential of Bacillus licheniformis for the production of 2,3-butanediol. J Ferment Bioeng 82:408–410
Perego P, Converti A, Del Borghi M (2003) Effects of temperature, inoculum size and starch hydrolyzate concentration on butanediol production by Bacillus licheniformis. Bioresour Technol 89:125–131
Syu MJ (2001) Biological production of 2,3-butanediol. Appl Microbiol Biotechnol 55:10–18
Voloch M, Jansen NB, Ladisch MR, Tsao GT, Narayan R, Rodwell VW (1985) 2,3-Butanediol. In: Moo-Young M, Blanch HW, Drew S, Wang DIC (eds) Comprehensive biotechnology. Pergamon, Oxford, pp 933–947
Wang Q, Chen T, Zhao X, Chamu J (2012) Metabolic engineering of thermophilic Bacillus licheniformis for chiral pure d-2,3-butanediol production. Biotechnol Bioeng 109:1610–1621
Yang T, Rao Z, Zhang X, Lin Q, Xia H, Xu Z, Yang S (2011) Production of 2,3-butanediol from glucose by GRAS microorganism Bacillus amyloliquefaciens. J Basic Microbiol 51:650–658
Zeng AP, Biebl H, Deckwer WD (1991) Production of 2,3-butanediol in a membrane bioreactor with cell recycle. Appl Microbiol Biotechnol 34:463–468
Zhang L, Ja S, Hao Y, Zhu J, Chu J, Wei D, Shen Y (2010a) Microbial production of 2,3-butanediol by a surfactant (serrawettin)-deficient mutant of Serratia marcescens H30. J Ind Microbiol Biotechnol 37:857–862
Zhang L, Yang Y, Ja S, Shen Y, Wei D, Zhu J, Chu J (2010b) Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30. Bioresour Technol 101:1961–1967
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This work was carried out in the framework of the European Research Area Network for Industrial Biotechnology (ERA-IB) and was funded by the Fachagentur Nachwachsende Rohstoffe e.V. (FNR) (grant no. 22009608A-E).
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Jurchescu, IM., Hamann, J., Zhou, X. et al. Enhanced 2,3-butanediol production in fed-batch cultures of free and immobilized Bacillus licheniformis DSM 8785. Appl Microbiol Biotechnol 97, 6715–6723 (2013). https://doi.org/10.1007/s00253-013-4981-z
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DOI: https://doi.org/10.1007/s00253-013-4981-z