Abstract
With the purpose of generating a microbial strain for l-ornithine production in Corynebacterium glutamicum, genes involved in the central carbon metabolism were inactivated so as to modulate the intracellular level of NADPH, and to evaluate their effects on l-ornithine production in C. glutamicum. Upon inactivation of the 6-phosphoglucoisomerase gene (pgi) in a C. glutamicum strain, the concomitant increase in intracellular NADPH concentrations from 2.55 to 5.75 mmol g−1 (dry cell weight) was accompanied by reduced growth rate and l-ornithine production, suggesting that l-ornithine production is not solely limited by NADPH availability. In contrast, inactivation of the gluconate kinase gene (gntK) led to a 51.8 % increase in intracellular NADPH concentration, which resulted in a 49.9 % increase in l-ornithine production. These results indicate that excess NADPH is not necessarily rate-limiting, but is required for increased l-ornithine production in C. glutamicum.
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Acknowledgments
This study was supported by the Advanced R&D supporting business between industry and University funded by the Small and Medium Business Administration, Republic of Korea, a grant from the Next-Generation BioGreen 21 Program (no. PJ0080992011), Rural Development Administration, Republic of Korea, and, in part, by the Sangji University Research Fund 2011.
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Hwang, GH., Cho, JY. Implication of gluconate kinase activity in l-ornithine biosynthesis in Corynebacterium glutamicum . J Ind Microbiol Biotechnol 39, 1869–1874 (2012). https://doi.org/10.1007/s10295-012-1197-7
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DOI: https://doi.org/10.1007/s10295-012-1197-7