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Effect of pyruvate dehydrogenase complex deficiency on l-lysine production with Corynebacterium glutamicum

  • Applied Genetics and Molecular Biotechnology
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Abstract

Intracellular precursor supply is a critical factor for amino acid productivity of Corynebacterium glutamicum. To test for the effect of improved pyruvate availability on l-lysine production, we deleted the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex (PDHC) in the l-lysine-producer C. glutamicum DM1729 and characterised the resulting strain DM1729-BB1 for growth and l-lysine production. Compared to the host strain, C. glutamicum DM1729-BB1 showed no PDHC activity, was acetate auxotrophic and, after complete consumption of the available carbon sources glucose and acetate, showed a more than 50% lower substrate-specific biomass yield (0.14 vs 0.33 mol C/mol C), an about fourfold higher biomass-specific l-lysine yield (5.27 vs 1.23 mmol/g cell dry weight) and a more than 40% higher substrate-specific l-lysine yield (0.13 vs 0.09 mol C/mol C). Overexpression of the pyruvate carboxylase or diaminopimelate dehydrogenase genes in C. glutamicum DM1729-BB1 resulted in a further increase in the biomass-specific l-lysine yield by 6 and 56%, respectively. In addition to l-lysine, significant amounts of pyruvate, l-alanine and l-valine were produced by C. glutamicum DM1729-BB1 and its derivatives, suggesting a surplus of precursor availability and a further potential to improve l-lysine production by engineering the l-lysine biosynthetic pathway.

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Acknowledgement

We thank Brigitte Bathe for providing C. glutamicum DM1729 and Lothar Eggeling for providing plasmids pJC33, pJC40 and pJC50. The support of the Fachagentur Nachwachsende Rohstoffe of the BMVEL (grant 04NR004/22000404) is gratefully acknowledged.

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Author notes

  1. Mark E. Schreiner

    Present address: R&D women’s health, Europe, Johnson & Johnson GmbH, 42289, Wuppertal, Germany

Authors and Affiliations

  1. Institute of Microbiology and Biotechnology, University of Ulm, 89069, Ulm, Germany

    Bastian Blombach, Mark E. Schreiner & Bernhard J. Eikmanns

  2. Institute of Biotechnology 2, Research Center Jülich, 52425, Jülich, Germany

    Matthias Moch & Marco Oldiges

Authors
  1. Bastian Blombach
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  2. Mark E. Schreiner
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  3. Matthias Moch
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  4. Marco Oldiges
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  5. Bernhard J. Eikmanns
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Corresponding author

Correspondence to Bernhard J. Eikmanns.

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This study is dedicated to Prof. Dr. Hermann Sahm on the occasion of his 65th birthday.

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Blombach, B., Schreiner, M.E., Moch, M. et al. Effect of pyruvate dehydrogenase complex deficiency on l-lysine production with Corynebacterium glutamicum . Appl Microbiol Biotechnol 76, 615–623 (2007). https://doi.org/10.1007/s00253-007-0904-1

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  • DOI: https://doi.org/10.1007/s00253-007-0904-1

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