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Increasing Pleurotus ostreatus laccase production by culture medium optimization and copper/lignin synergistic induction

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Laccases have great biotechnological potential in diverse industries as they catalyze the oxidation of a broad variety of chemical compounds. Production of laccases by basidiomycetes has been broadly studied as they secrete the enzymes, grow on cheap substrates, and they generally produce more than one isoenzyme (constitutive and/or inducible). Laccase production and isoenzyme profile can be modified through medium composition and the use of inducers. The objective of this work was to increase laccase production by Pleurotus ostreatus CP-50 through culture medium optimization and the simultaneous use of copper and lignin as inducers. Increased fungal growth was obtained through the use of a factorial fractional experimental design 26–2 where the influence of the nature and concentration of carbon and nitrogen sources was assessed. Although specific laccase production (U/mg biomass) decreased when malt extract medium was supplemented with carbon and nitrogen sources, fungal growth and laccase volumetric activity increased four and sixfold, respectively. The effect of media supplementation with copper and/or lignin on laccase production by P. ostreatus CP-50 was studied. A positive synergistic effect between copper and lignin was observed on laccase production. Overall, the use of an optimized medium and the simultaneous addition of copper and lignin improved growth, laccase volumetric activity, and process productivity by 4-, 60-, and 10-fold, respectively.

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Acknowledgments

This study was financed by DGAPA-UNAM (IN217909). We thank M. Caro, V. Albiter and M. García for their technical assistance. The authors also thank J.M. Hurtado for computer support.

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Authors and Affiliations

  1. Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, 62250, Morelos, Mexico

    Raunel Tinoco, Abisaí Acevedo, Enrique Galindo & Leobardo Serrano-Carreón

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  1. Raunel Tinoco
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  2. Abisaí Acevedo
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  3. Enrique Galindo
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  4. Leobardo Serrano-Carreón
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Correspondence to Leobardo Serrano-Carreón.

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Tinoco, R., Acevedo, A., Galindo, E. et al. Increasing Pleurotus ostreatus laccase production by culture medium optimization and copper/lignin synergistic induction. J Ind Microbiol Biotechnol 38, 531–540 (2011). https://doi.org/10.1007/s10295-010-0797-3

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  • DOI: https://doi.org/10.1007/s10295-010-0797-3

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