Skip to main content

Advertisement

SpringerLink
Log in

Effects of fluoride in bone repair: an evaluation of RANKL, OPG and TRAP expression

  • Original Article
  • Published:
Odontology Aims and scope Submit manuscript

Abstract

The objective of this study was to evaluate comparatively the effect of fluoride in the expression of the receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG) and tartrate-resistant acid phosphatase (TRAP) in alveolar bone repair in rats. We used 3 groups of male Wistar rats (n = 5/group), which received drinking water containing different doses of F (NaF): 0, 5 and 50 ppm, for 60 days before the incisors extraction. The upper incisors were extracted and the animals were killed 7, 14, 21 and 30 days after extraction. The hemi-maxillae were collected for microscopic examination (histomorphometric and immunostaining for RANKL, OPG and TRAP). Histomorphometric analysis confirmed an increase in the volume density of neoformed bone between 7 and 30 days for groups control, 5 and 50 ppm of F, with a concomitant decrease in the volume density of connective tissue and blood clot. Higher blood clot for groups 5 and 50 ppm of F at 30 days was observed. The RANKL and OPG expressions were not changed by chronic exposure to fluoride in the drinking water during the studied periods; on the other hand, TRAP expression was changed (at 7 days) by chronic exposure to fluoride (p < 0.05). It was concluded that F in high concentrations can slow the blood clot remission and bone repair, and alter the TRAP expression in the beginning of the bone tissue repair. However, a better understanding about this blood clot remission phenomenon is required.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Lau KH, Baylink DJ. Phosphotyrosyl protein phosphatases: potential regulators of cell proliferation and differentiation. Crit Rev Oncog. 1993;4:451–71.

    PubMed  Google Scholar 

  2. Ohmi K, Nakagaki H, Tsuboi S, Okumura A, Sugiyama T, Thuy TT, Robinson C. The effect of fluoridation and its discontinuation on fluoride profiles in the alveolar bone of rat. Calcif Tissue Int. 2005;77:226–32.

    Article  PubMed  Google Scholar 

  3. Cadir B, Kürkcü M, Öz IA, Benliday ME. Effects of vitamin K1 on fluoride-induced bone changes in growing rats: a histomorphometric and radiodensitometric study. Arch Oral Biol. 2009;54:512–7.

    Article  PubMed  Google Scholar 

  4. Qu WJ, Zhong DB, Wu PF, Wang JF, Han B. Sodium fluoride modulates caprine osteoblast proliferation and differentiation. J Bone Miner Metab. 2008;26:328–34.

    Article  PubMed  Google Scholar 

  5. Qu H, Wei M. The effect of fluoride contents in fluoridated hydroxyapatite on osteoblast behavior. Acta Biomater. 2006;2:113–9.

    Article  PubMed  Google Scholar 

  6. Cooper LF, Zhou Y, Takebe J, Guo J, Abron A, Holmén A, Ellingsen JE. Fluoride modification effects on osteoblast behavior and bone formation at TiO2 grit-blasted c.p. titanium endosseous implants. Biomaterials. 2006;27:926–36.

    Article  PubMed  Google Scholar 

  7. Kürkcü M, Benlidayi ME, Ozsoy S, Ozyeğin LS, Oktar FN, Kurtoğlu C. Histomorphometric evaluation of implants coated with enamel or dentine derived fluoride-substituted apatite. J Mater Sci Mater Med. 2007;9:59–65.

    Google Scholar 

  8. Collaert B, Wijnen L, De Bruyn H. A 2-year prospective study on immediate loading with fluoride-modified implants in the edentulous mandible. Clin Oral Implants Res. 2011;18:1–6.

    Google Scholar 

  9. Haguenauer D, Welch V, Shea B, Tugwell P, Adachi JD, Wells G. Fluoride for the treatment of postmenopausal osteoporotic fractures: a meta-analysis. Osteoporos Int. 2000;11:727–38.

    Article  PubMed  Google Scholar 

  10. Shashi A, Kumar M, Bhardwaj M. Incidence of skeletal deformities in endemic fluorosis. Trop Doct. 2008;38:231–3.

    Article  Google Scholar 

  11. Tamer MN, Kale Koroglu B, Arslan C, Akdogan M, Koroglu M, Cam H, Yildiz M. Osteosclerosis due to endemic fluorosis. Sci Total Environ. 2007;373:43–8.

    Article  PubMed  Google Scholar 

  12. Oruc N. Occurrence and problems of high fluoride waters in Turkey: an overview. Environ Geochem Health. 2008;30:315–23.

    Article  PubMed  Google Scholar 

  13. Von Tirpitz C, Klaus J, Steinkamp M, Hofbauer LC, Kratzer W, Mason R, Boehm BO, Adler G, Reinshagen M. Therapy of osteoporosis in patients with Crohn’s disease: a randomized study comparing sodium fluoride and ibandronate. Aliment Pharmacol Ther. 2003;17:807–16.

    Article  Google Scholar 

  14. Yan D, Gurumurthy A, Wright M, Pfeiler TW, Loboa EG, Everett ET. Genetic background influences fluoride’s effects on osteoclastogenesis. Bone. 2007;41:1036–44.

    Article  PubMed Central  PubMed  Google Scholar 

  15. Reeves PG, Nielsen FH, Fahey GC. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993;123:1939–51.

    PubMed  Google Scholar 

  16. Oliveira RC, Oliveira FH, Cestari TM, Taga R, Granjeiro JM. Morphometric evaluation of the repair of critical-size defects using demineralized bovine bone and autogenous bone grafts in rat calvaria. Clin Oral Implants Res. 2008;19:749–54.

    Article  PubMed  Google Scholar 

  17. Okamoto T, Russo MC. Wound healing following tooth extraction. Histochemical study in rats. Rev Fac Odontol Aracatuba. 1973;2:153–69.

    PubMed  Google Scholar 

  18. Weibel ER. Stereological principles for morphometry in electron microscopic cytology. Int Rev Cytol. 1969;26:235–302.

    PubMed  Google Scholar 

  19. Hallanger Johnson JE, Kearns AE, Doran PM, Khoo TK, Wermers RA. Fluoride-related bone disease associated with habitual tea consumption. Mayo Clin Proc. 2007;82:719–24.

    PubMed  Google Scholar 

  20. Grobler SR, Louw AJ, Chikte UME, Rossouw RJ, Van TJ, Kotze W. The relationships between two different drinking water fluoride levels, dental fluorosis and bone mineral density of children. Open Dent J. 2009;3:48–54.

    Article  PubMed Central  PubMed  Google Scholar 

  21. Raffi MB, Méndez MC, Riet-Correa F. Histomorphometric and histological evaluations of the bone lesions caused by fluoride in chickens (in Portuguese). Pesq Vet Bras. 1997;17:69–76.

    Article  Google Scholar 

  22. Kragstrup J, Richards A, Fejerskov O. Experimental osteo-fluorosis in the domestic pig: a histomorphometric study of vertebral trabecular bone. J Dent Res. 1984;63:885–9.

    Article  PubMed  Google Scholar 

  23. Carvalho JG, Cestari TM, Oliveira RC, Buzalaf MA. Fluoride effects on ectopic bone formation in young and old rats. Methods Find Exp Clin Pharmacol. 2008;30:287–94.

    Article  PubMed  Google Scholar 

  24. Urist MR. Bone: formation by autoinduction. Science. 1965;150:893–9.

    Article  PubMed  Google Scholar 

  25. Librus H, Pietrokovski J, Ulmanski M, Gedalia I. The effect of fluoride on molar socket healing in the rat. Arch Oral Biol. 1973;18:1283–9.

    Article  PubMed  Google Scholar 

  26. Yan X, Feng C, Chen Q, Li W, Wang H, Lv L, Smith GW, Wang J. Effects of sodium fluoride treatment in vitro on cell proliferation, apoptosis and caspase-3 and caspase-9 mRNA expression by neonatal rat osteoblasts. Arch Toxicol. 2009;83:451–8.

    Article  PubMed  Google Scholar 

  27. Mousny M, Omelon S, Wise L, Everett ET, Dumitriu M, Holmyard DP, Banse X, Devogelaer JP, Grynpas MD. Fluoride effects on bone formation and mineralization are influenced by genetics. Bone. 2008;43:1067–74.

    Article  PubMed Central  PubMed  Google Scholar 

  28. Muller P, Schmid K, Warnecke G, Setnikar I, Simon B. Sodium fluoride-induced gastric mucosal lesions: comparison with sodium monofluorophosphate. Z Gastroenterol. 1992;30:252–4.

    PubMed  Google Scholar 

  29. Partanen S. Inhibition of human renal acid phosphatases by nephrotoxic micromolar concentrations of fluoride. Exp Toxicol Pathol. 2002;54:231–7.

    Article  PubMed  Google Scholar 

  30. Yan X, Yan X, Morrison A, Han T, Chen Q, Li J, Wang J. Fluoride induces apoptosis and alters collagen I expression in rat osteoblasts. Toxicol Lett. 2011;200:133–8.

    Article  PubMed  Google Scholar 

  31. Wang Z, Yang X, Yang S, Ren G, Ferreri M, Su Y, Chen L, Han B. Sodium fluoride suppress proliferation and induce apoptosis through decreased insulin-like growth factor-I expression and oxidative stress in primary cultured mouse osteoblasts. Arch Toxicol. 2011. doi:10.1007/s00204-011-0697-y.

    Google Scholar 

  32. Willems HM, van den Heuvel EG, Castelein S, Buisman JK, Bronckers AL, Bakker AD, Klein-Nulend J. Fluoride inhibits the response of bone cells to mechanical loading. Odontology. 2011;99:112–8.

    Article  PubMed  Google Scholar 

  33. Hars E, Massler M. Effects of fluorides, cortico-steroids and tetracyclines on extraction wound healing in rats. Acta Odontol Scand. 1972;30:511–22.

    Article  PubMed  Google Scholar 

  34. Chou M-Y, Yan D, Jafarov T, Everett ET. Modulation of murine bone marrow-derived CFU-F and CFU-OB by in vivo bisphosphonate and fluoride treatments. Orthod Craniofac Res. 2009;12:141–7.

    Article  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

We appreciate the technical collaboration of Danielle Santi Ceolin laboratory (Laboratory of Histology), and student Jaqueline Caetano Faria. Fundação de Amparo a Pesquisa do Estado de São Paulo (Process: 2006/06430-3, 2007/00494-2 and 2008/09926-5) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (Process: 472798/2008-1).

Author information

Authors and Affiliations

  1. Department of Biological Sciences, Bauru Dental School, University of São Paulo (USP), Alameda Dr. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo (SP), 17012-901, Brazil

    Mileni da Silva Fernandes, Marcela Mitsuko Yanai, Gisele Miyamura Martins, Flávia Godoy Iano, Aline Lima Leite, Tânia Mary Cestari, Rumio Taga, Marília Afonso Rabelo Buzalaf & Rodrigo Cardoso de Oliveira

Authors
  1. Mileni da Silva Fernandes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marcela Mitsuko Yanai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gisele Miyamura Martins
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Flávia Godoy Iano
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Aline Lima Leite
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tânia Mary Cestari
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Rumio Taga
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Marília Afonso Rabelo Buzalaf
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Rodrigo Cardoso de Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rodrigo Cardoso de Oliveira.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fernandes, M.S., Yanai, M.M., Martins, G.M. et al. Effects of fluoride in bone repair: an evaluation of RANKL, OPG and TRAP expression. Odontology 102, 22–30 (2014). https://doi.org/10.1007/s10266-012-0083-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10266-012-0083-0

Keywords

Search

Navigation