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The Effect of Fluoridation and Its Discontinuation on Fluoride Profiles in the Alveolar Bone of Rat

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Abstract

We investigated the effect of fluoridation and its discontinuation on fluoride content in the alveolar portion of the mandible in rats. Drinking water with three different fluoride contents (0, 50, 100 ppmF) was given to rats for three different periods (4, 13 and 25 weeks). Fluoride concentrations were measured in the crest, the middle, and the apical parts of the alveolar bone and in the body of the mandible. Furthermore, after fluoridated drinking water was given to rats for 4 or 13 weeks, distilled water was given to them for 21 or 12 weeks respectively; and the effect of the discontinuation on fluoride profiles was investigated. Layer samples were analyzed by abrasive microsampling. Fluoride and phosphorus concentrations were determined by ion-specific electrode and colorimetric procedures, respectively. There was an increase in fluoride concentrations in the mandible in proportion to the fluoride content in the drinking water and the duration of fluoridation. After fluoridation was discontinued, fluoride concentrations in the surface layers of the mandible presented a decrease. Among the four different parts of the mandible, the upper part of the alveolar bone and the alveolar crest part presented the highest rates of reduction. The relative reduction rate of fluoride concentration was closely related to the duration of discontinuation. The alveolar crest was affected most by the discontinuation of fluoridation, presenting the greatest reduction.

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Acknowledgments

Part of this work was supported by KAKENHI(A)No17209066.

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Authors and Affiliations

  1. Depertment of Preventive Dentistry and Dental Public Health, School of Dentistry, Aichi-Gakuin University, 1-100 Kusumoto-cho, Nagoya, 464-8650, Japan

    Kyohei Ohmi, Haruo Nakagaki, Shinji Tsuboi, Akihiro Okumura, Tomoko Sugiyama & Tran Thu Thuy

  2. Division of Oral Biology, Leeds Dental Institute, University of Leeds, Clarendon Way, Leeds, LS2 9LU, United Kingdom

    Colin Robinson

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  1. Kyohei Ohmi
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  2. Haruo Nakagaki
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  3. Shinji Tsuboi
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  4. Akihiro Okumura
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  5. Tomoko Sugiyama
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  6. Tran Thu Thuy
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  7. Colin Robinson
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Correspondence to Kyohei Ohmi.

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Ohmi, K., Nakagaki, H., Tsuboi, S. et al. The Effect of Fluoridation and Its Discontinuation on Fluoride Profiles in the Alveolar Bone of Rat. Calcif Tissue Int 77, 226–232 (2005). https://doi.org/10.1007/s00223-004-1304-5

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  • DOI: https://doi.org/10.1007/s00223-004-1304-5

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