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Chromosome banding and FISH with rDNA probe in the diploid and tetraploid loach Misgurnus anguillicaudatus

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Abstract

The chromosomes of the diploid and tetraploid loach Misgurnus anguillicaudatus were analyzed by staining with Ag, chromomycin A3 (CMA3)/distamycin A (DA), and DA/4′,6-diamidino-2-phenylindole (DAPI), and using fluorescence in situ hybridization (FISH) with 5.8S + 28S rDNA as a probe. Nucleolus organizer regions (NORs) were mapped to the telomeric region of the short arms of the largest (first) metacentric chromosome pair in the diploid loach with 2n = 50 and the homologous quartet in the tetraploid loach with 4n = 100. The NORs were positive at the same region of the first metacentric chromosome for Ag and CMA3/DA stainings, but negative for DA/DAPI staining. Four signals at the homologs within the same quartet suggest the duplication of the entire genome from diploid to tetraploid status. However, a size difference was detected between the rDNA signals by FISH and CMA3 banding.

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Acknowledgments

This study was partly supported by Grants-in-Aid for the 21st Century COE (Center of Excellence) Program (K02, 2004-2008 FY) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan and for Scientific Research (B) (No. 18380138, 2006-2008FY and No. 21380114, 2009-2011FY) from the Japan Society for the Promotion of Science (JSPS) to K.A. Part of this work was also supported by the JSPS Ronpaku (Dissertation Ph.D.) Program (CSC-10610) to Y.-J.L.

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Authors and Affiliations

  1. Life Science and Technology Institute, Dalian Ocean University, Dalian, 116023, People’s Republic of China

    Ya-Juan Li, Yi Tian, Ming-Zhao Zhang, Ping-Ping Tian & Zhuo Yu

  2. Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, 041-8611, Japan

    Syuiti Abe & Katsutoshi Arai

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  1. Ya-Juan Li
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Correspondence to Katsutoshi Arai.

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Li, YJ., Tian, Y., Zhang, MZ. et al. Chromosome banding and FISH with rDNA probe in the diploid and tetraploid loach Misgurnus anguillicaudatus . Ichthyol Res 57, 358–366 (2010). https://doi.org/10.1007/s10228-010-0168-0

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