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Distribution and reproductive capacity of natural triploid individuals and occurrence of unreduced eggs as a cause of polyploidization in the loach,Misgurnus anguillicaudatus

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Abstract

Loaches (Misgurnus anguillicaudatus) were collected from 35 localities in Japan and assayed by flow cytometry to determine ploidy status. No tetraploids were found, with samples from 33 localities having no or few (1.2–3.2%) triploids. Samples collected from Ichinomiya Town, Aichi Prefecture, showed a relatively high rate of triploidy (7.7%). Samples collected from a fish farm in Hirokami Village, Niigata Prefecture, also showed high proportions of triploids (2.0–15.8%), these triploid males being sterile, but the females producing both large-sized triploid and small-sized haploid eggs. Such eggs developed bisexually rather than gynogenetically, giving rise to viable tetraploid and diploid offspring after normal fertilization. Of eight diploid females obtained from the same locality, one produced a high incidence of viable diploid gynogens (55%) after gynogenetic induction by fertilization with UV-irradiated spermatozoa. These observations indicated the presence of diploid fish which produced both diploid and haploid eggs. Thus, triploid and diploid individuals were also produced after fertilization with haploid spermatozoa. These results suggested that the occurrence of such unreduced eggs may be a cause of natural polyploidization in this species.

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Author notes

  1. Quanqi Zhang

    Present address: National Research Institute of Aquaculture, Tamaki, 519-0413, Mie, Japan

  2. Katsutoshi Arai

    Present address: Faculty of Fisheries, Hokkaido University, Hakodate, 041-8611, Hokkaido, Japan

Authors and Affiliations

  1. Faculty of Applied Biological Science, Hiroshima University, Higashi-hiroshima, 739-8528, Hiroshima, Japan

    Quanqi Zhang & Katsutoshi Arai

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  1. Quanqi Zhang
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  2. Katsutoshi Arai
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Correspondence to Katsutoshi Arai.

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Zhang, Q., Arai, K. Distribution and reproductive capacity of natural triploid individuals and occurrence of unreduced eggs as a cause of polyploidization in the loach,Misgurnus anguillicaudatus . Ichthyological Research 46, 153–161 (1999). https://doi.org/10.1007/BF02675433

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  • DOI: https://doi.org/10.1007/BF02675433

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