Abstract
Cytoplasmic male sterility (CMS) has been widely used in crop cross breeding. There has been much research on wheat CMS. However, the correlation between S-type CMS and mitochondrial genome remains elusive. Herein, we sequenced the mitochondrial genome of wheat CMS line and compared it with the maintainer line. The results showed that the mitochondrial genome of CMS line encoded 26 tRNAs, 8 rRNAs, and 35 protein-coding genes, and the cob encoding complex III in which the protein coding gene is mutated. This protein is known to affect reactive oxygen (ROS) production. The analysis of ROS metabolism in developing anthers showed that the deficiency of antioxidants and antioxidant enzymes in the sterile system aggravated membrane lipid oxidation, resulting in ROS accumulation, and influencing the anther development. Herein, cob is considered as a candidate causative gene sequence for CMS.
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The authors are grateful to all lab members for their useful suggestions, support, and encouragement.
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This work was supported by the Natural Science Foundation of Anhui Province (1708085QC62); Excellent Talents Support Program of Colleges and Universities in Anhui Province (gxyq2020014); Scientific Research Foundation of the Higher Education Institutions of Anhui Province, China (KJ2019A585, KJ2019A0961); Anhui University Collaborative Innovation Project (GXXT-2019–033); and the Doctoral Scientific Research Foundation of Huaibei Normal University (15601047).
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Resources: RW and LZ. Methodology: RW and WW. Formal analysis: QB and RW. Writing—original draft preparation: RW and QB. Writing—review and editing: QB, PZ, and GL. Supervision: QB. Funding acquisition: QB. All authors have read and agreed to the published version of the manuscript.
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Wang, R., Ba, Q., Zhang, L. et al. Comparative analysis of mitochondrial genomes provides insights into the mechanisms underlying an S-type cytoplasmic male sterility (CMS) system in wheat (Triticum aestivum L.). Funct Integr Genomics 22, 951–964 (2022). https://doi.org/10.1007/s10142-022-00871-7
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DOI: https://doi.org/10.1007/s10142-022-00871-7