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Metabolism of reactive oxygen species in cotton cytoplasmic male sterility and its restoration

  • Physiology and Biochemistry
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Abstract

To elucidate reactive oxygen species (ROS) metabolism of cotton cytoplasmic male sterility and the effects of restorer gene on the metabolism of ROS, the metabolism changes in the production and scavenging of ROS and gene expression related to ROS-scavenging enzymes were investigated in the anther mitochondria of CMS line, maintainer line and hybrid F1. During the abortion preliminary stage (sporogenous cell division stage), anthers of CMS line had a little higher superoxide (O −2 ) production rate and hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents than those of maintainer or hybrid F1. Simultaneously, a little higher ROS contents might serve as a signal to increase the activity of superoxide dismutase (SOD) in anthers of CMS line to reduce the ROS damage to the anther development. But at the abortion peak (pollen mother cell meiosis stage), anthers of CMS line had extraordinarily higher ROS contents and lower ROS-scavenging enzymic activities compared with the hybrid F1, during which the ROS contents and ROS-scavenging enzymic activities in hybrid F1 were approximate to those of maintainer line. The expression of Mn-sod and apx mRNA in anther of CMS line was obviously inhibited when ROS produced with a great deal during anther abortion, however the gene expression in hybrid F1 kept normal with the maintainer. Excessive accumulation of O ·−2 , H2O2 and MDA, significant reduction of ROS-scavenging enzymic activities and lower gene expression level of ROS-scavenging enzyme were coinstantaneous with male cells death in anthers of CMS line. But when the restorer gene was transferred into CMS line, excessive production of ROS could be eliminated in the anthers of hybrid F1. The restorer gene likely plays an important role in keeping the dynamic balance between the production and elimination of ROS.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (863 Program: 2004AA212104, 973 Program: 2004CB11730502-1) and Science and Technology Research Program of Zhejiang Province in China (2005C22G2010011).

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Authors and Affiliations

  1. Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China

    Peidong Jiang, Xiaoquan Zhang, Yunguo Zhu, Wei Zhu, Haiyan Xie & Xuede Wang

  2. College of Life Science and Technology, Tongji University, Shanghai, 200092, China

    Yunguo Zhu

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  1. Peidong Jiang
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  2. Xiaoquan Zhang
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  3. Yunguo Zhu
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  4. Wei Zhu
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  5. Haiyan Xie
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  6. Xuede Wang
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Correspondence to Xuede Wang.

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Communicated by W.T. Kim.

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Jiang, P., Zhang, X., Zhu, Y. et al. Metabolism of reactive oxygen species in cotton cytoplasmic male sterility and its restoration. Plant Cell Rep 26, 1627–1634 (2007). https://doi.org/10.1007/s00299-007-0351-6

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  • DOI: https://doi.org/10.1007/s00299-007-0351-6

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