Abstract
The gibberellin biosynthesis pathway is well defined in Arabidopsis and features seven key enzymes including ent-copalyl diphosphate synthase (CPS), ent-kaurene synthase (KS), ent-kaurene oxidase (KO), ent-kaurenoic acid oxidase (KAO), GA 20-oxidase, GA 3-oxidase, and GA 2-oxidase. The Arabidopsis genes were used to identify their counterparts in wheat and the TaCPS, TaKS, TaKO, and TaKAO genes were cloned from Chinese Spring wheat. In order to determine their chromosome locations, expression patterns and feedback regulations, three TaCPS genes, three TaKS genes, three TaKO genes, and three TaKAO genes were cloned from Chinese Spring wheat. They are mainly located on chromosomes 7A, 7B, 7D and 2A, 2B and 2D. The expression patterns of TaCPS, TaKS, TaKO, and TaKAO genes in wheat leaves, young spikes, peduncles, the third and forth internodes were investigated using quantitative PCR. The results showed that all the genes were constitutively expressed in wheat, but their relative expression levels varied in different tissues. They were mainly transcribed in stems, secondly in leaves and spikes, and the least in peduncles. Feedback regulation of the TaCPS, TaKS, TaKO, and TaKAO genes was not evident. These results indicate that all the genes and their homologs may play important roles in the developmental processes of wheat, but each of the homologs may function differently in different tissues or during different developmental stages.
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This work was supported by the National Natural Science Foundation of China (90717118 and 30521001).
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Authors Yuanyuan Huang and Wenlong Yang contributed equally to this work.
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Additional file 1
Nucleotide sequence alignments of three homologs of TaKS, TaKO, and TaKAO. a TaKS, b TaKO, c TaKAO (PDF 2,866 kb)
Additional file 2
Chromosomal localization of TaCPS, TaKS, TaKO, and TaKAO. a The three homologs of TaCPS are located on chromosomes 7A, 7B, and 7D; b The three homologs of TaKS are located on chromosomes 2A, 2B, and 2D; c The three homologs of TaKO are located on chromosomes 7A, 7B, and 7D; d The three homologs of TaKAO are located on chromosomes 4A, 7A, and 7D (PDF 1,299 kb)
Additional file 3
Feedback regulation of TaCPS, TaKS, TaKO, and TaKAO in wheat treated with PAC or GA3, with H2O treatment as a control. a TaCPS, b TaKS, c TaKO, d TaKAO (PDF 406 kb)
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Huang, Y., Yang, W., Pei, Z. et al. The genes for gibberellin biosynthesis in wheat. Funct Integr Genomics 12, 199–206 (2012). https://doi.org/10.1007/s10142-011-0243-2
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DOI: https://doi.org/10.1007/s10142-011-0243-2