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Identification of transposons, retroelements, and a gene family predominantly expressed in floral tissues in chromosome 3DS of the hexaploid wheat progenitor Aegilops tauschii

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Abstract

A multigene family expressed during early floral development was identified on the short arm of wheat chromosome 3D in the region of the Ph2 locus, a locus controlling homoeologous chromosome pairing in allohexaploid wheat. Physical, genetic and molecular characterisation of the Wheat Meiosis 1 (WM1) gene family identified seven members that localised within a region of 173-kb. WM1 gene family members were sequenced and they encode mainly type Ia plasma membrane-anchored leucine rich repeat-like receptor proteins. In situ expression profiling suggests the gene family is predominantly expressed in floral tissue. In addition to the WM1 gene family, a number of other genes, gene fragments and pseudogenes were identified. It has been predicted that there is approximately one gene every 19-kb and that this region of the wheat genome contains 23 repetitive elements including BARE-1 and Wis2-1 like sequences. Nearly 50% of the repetitive elements identified were similar to known transposons from the CACTA superfamily. Ty1-copia, Ty3-gypsy and Athila LTR retroelements were also prevalent within the region. The WM1 gene cluster is present on 3DS and on barley 3HS but missing from the A and B genomes of hexaploid wheat. This suggests either recent generation of the cluster or specific deletion of the cluster during wheat polyploidisation. The evolutionary significance of the cluster, its possible roles in disease response or floral and early meiotic development and its location at or near the Ph2 locus are discussed.

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Abbreviations

LRR:

Leucine-rich repeat

RLKs:

Receptor-like kinases

RLPs:

Receptor-like proteins

ORF:

Open reading frame

Ph :

Pairing homoeologous

TtBAC :

Aegilops tauschii bacterial artificial chromosome

WM :

Wheat meiosis

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Acknowledgements

The authors thank Dr. Thomas Wicker of the Institute of Plant Biology, University of Zürich for sequence analysis support. RW was funded through GRDC with additional support from Agriculture Western Australia. The authors gratefully acknowledge the Molecular Plant Breeding Cooperative Research Centre and the Australian Research Council (Special Research Centre for Basic and Applied Plant Molecular Biology), for funding this research.

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Authors and Affiliations

  1. Molecular Plant Breeding Cooperative Research Centre, School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, PMB1, Glen Osmond, 5064, South Australia, Australia

    Ryan Whitford & Jason A. Able

  2. DuPont Agricultural Biotechnology-Genomics, Delaware Technology Park, Suite 200, 1 Innovation Way, P.O. Box 6104, Newark, DE, 19714-6104, USA

    Luke Gumaelius, Petra Wolters & Scott Tingey

  3. Australian Centre of Plant Functional Genomics, School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, PMB1, Glen Osmond, 5064, South Australia, Australia

    Ute Baumann, Tim Sutton & Peter Langridge

  4. Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, Technology Park 927, 9052, Ghent, Belgium

    Ryan Whitford

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Correspondence to Jason A. Able.

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Whitford, R., Baumann, U., Sutton, T. et al. Identification of transposons, retroelements, and a gene family predominantly expressed in floral tissues in chromosome 3DS of the hexaploid wheat progenitor Aegilops tauschii . Funct Integr Genomics 7, 37–52 (2007). https://doi.org/10.1007/s10142-006-0026-3

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