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Can photobiomodulation associated with implantation of mesenchymal adipose-derived stem cells attenuate the expression of MMPs and decrease degradation of type II collagen in an experimental model of osteoarthritis?

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Abstract

This study aimed to determine whether photobiomodulation therapy (PBMT) could improve the bioavailability and chondroprotective benefits of mesenchymal stem cells injected into the knees of rats used as an experimental model of osteoarthritis (OA) as well as reduce the expression of matrix metalloproteinases (MMPs) and degradation of type II collagen (COL2-1) in the cartilage. Adipose-derived stem/stromal cells (ADSCs) were collected from three male Fischer 344 rats and characterized by flow cytometry. Fifty female Fischer 344 rats were distributed into five groups of 10 animals each. These groups were as follows: control, OA, OA PBMT, OA ADSC, and OA ADSC PBMT. OA was induced in the animals using a 4% papain solution. Animals from the OA ADSC and OA ADSC PBMT groups received an intra-articular injection of 10 × 106 ADSCs and were treated with PBMT by irradiation (wavelength: 808 nm, power: 50 mW, energy: 42 J, energy density: 71.2 J/cm2, spot size: 0.028). Euthanasia was performed 7 days after the first treatment. The use of PBMT alone and the injection of ADSCs resulted in downregulation of pro-inflammatory cytokines and MPs in cartilage compared to the OA group. PBMT and ADSCs caused upregulation of tissue inhibitors of MPs 1 and 2 and mRNA and protein expression of COL2-1 in cartilage compared to the OA group. The intra-articular injection of ADSCs and PBMT prevented joint degeneration resulting from COL2-1 degradation and modulated inflammation by downregulating cytokines and MMPs in the OA group.

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Funding

The authors gratefully acknowledge the Master’s scholarships provided to Tatiane Garcia Stancker by FAPESP (grant number 2015/13656-7). This work was supported by grants from the São Paulo Research Foundation (FAPESP, grant number (2015/13677-4) and the National Council for Scientific and Technological (grant number 309065/2015-1).

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Authors and Affiliations

  1. Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 235, São Paulo, SP, Brazil

    Tatiane Garcia Stancker, Solange Almeida dos Santos, Marcia Ataize dos Santos Vieira, Maíra Cecília Brandão Simões, Ernesto Cesar Leal-Junior & Paulo de Tarso Camillo de Carvalho

  2. Postgraduate Program in Biophotonics, Universidade Nove de Julho (UNINOVE), São Paulo, SP, Brazil

    Andrey Jorge Serra, Rafael do Nascimento Lima & Paulo de Tarso Camillo de Carvalho

  3. Postgraduate Program in Medicine, Universidade Nove de Julho (UNINOVE), São Paulo, SP, Brazil

    Regiane dos Santos Feliciano & José Antônio Silva Jr.

  4. Postgraduate Program in Cardiology, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil

    Stella Souza Vieira

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  1. Tatiane Garcia Stancker
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Correspondence to Paulo de Tarso Camillo de Carvalho.

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The Ethics Committee of the Federal University of São Paulo (UNIFESP, opinion CEUA no. 4987090316) approved all experimental procedures. All procedures were performed in accordance with the International Council for Laboratory Animal Science.

Conflict of interest

Professor Ernesto Cesar Pinto Leal-Junior receives research support from Multi Radiance Medical (Solon, OH, USA), a laser device manufacturer. The remaining authors declare that they have no conflict of interests.

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Stancker, T.G., Vieira, S.S., Serra, A.J. et al. Can photobiomodulation associated with implantation of mesenchymal adipose-derived stem cells attenuate the expression of MMPs and decrease degradation of type II collagen in an experimental model of osteoarthritis?. Lasers Med Sci 33, 1073–1084 (2018). https://doi.org/10.1007/s10103-018-2466-0

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