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Effects of low level laser therapy on attachment, proliferation, and gene expression of VEGF and VEGF receptor 2 of adipocyte-derived mesenchymal stem cells cultivated under nutritional deficiency

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Abstract

Low-level laser therapy (LLLT) has been shown to increase the proliferation of several cell types. We evaluated the effects of LLLT on adhesion, proliferation, and gene expression of vascular endothelial growth factor (VEGF) and type 2 receptor of VEGF (VEGFR2) at mesenchymal stem cells (MSCs) from human (hMSCs) and rat (rMSCs) adipose tissues on nutritional deficiencies. A dose-response curve was performed with cells treated with laser Ga-Al-As (660 nm, 30 mW) at energy of 0.7 to 9 J. Cell adhesion and proliferation were quantified 20, 40, and 60 min after LLLT and 24, 72, and 120 h after cultivation. Gene expression was verified by RT-PCR after 2 h of LLLT. A minor nutritional support caused a significant decrease in proliferation and adhesion of hMSCs and rMSCs. However, at the lowest LLLT dose (0.7 J), we observed a higher proliferation in hMSCs at standard condition shortly after irradiation (24 h). Adhesion was higher in hMSCs cultivated in controlled conditions at higher LLLT doses (3 and 9 J), and rMSCs show a reduction in the adhesion on 1.5 to 9 J. On nutritional deprivation, a 9 J dose was shown to reduce proliferation with 24 h and adhesion to all culture times in rMSCs. VEGF and VEGFR2 were increased after LLLT in both cell types. However, hMSCs under nutritional deprivation showed higher expression of VEGF and its receptor after irradiation with other laser doses. In conclusion, LLLT on human and rat MSCs might upregulate VEGF messenger RNA (mRNA) expression and modulate cell adhesion and proliferation distinctively.

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Authors and Affiliations

  1. Nove de Julho University, Rua Vergueiro, 235, Liberdade, 01504-001, São Paulo, SP, Brazil

    Tabata Santos de Oliveira, Andrey Jorge Serra, Martha Trindade Manchini, Paulo de Tarso Camillo de Carvalho & José Antônio Silva Jr

  2. São Paulo University–InCor, São Paulo, SP, Brazil

    Vinicius Bassaneze & José Eduardo Krieger

  3. Federal University of São Paulo, São Paulo, SP, Brazil

    Daniela Espindola Antunes & Paulo José Ferreira Tucci

  4. São Judas Tadeu University, São Paulo, SP, Brazil

    Danilo Sales Bocalini

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  1. Tabata Santos de Oliveira
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  2. Andrey Jorge Serra
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  3. Martha Trindade Manchini
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  4. Vinicius Bassaneze
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  5. José Eduardo Krieger
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  6. Paulo de Tarso Camillo de Carvalho
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  9. Paulo José Ferreira Tucci
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  10. José Antônio Silva Jr
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Correspondence to Andrey Jorge Serra.

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de Oliveira, T.S., Serra, A.J., Manchini, M.T. et al. Effects of low level laser therapy on attachment, proliferation, and gene expression of VEGF and VEGF receptor 2 of adipocyte-derived mesenchymal stem cells cultivated under nutritional deficiency. Lasers Med Sci 30, 217–223 (2015). https://doi.org/10.1007/s10103-014-1646-9

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  • DOI: https://doi.org/10.1007/s10103-014-1646-9

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