Abstract
This study aimed to systematically assess the parameter-specific effects of the diode low-level laser on human gingival fibroblasts (HGFs) and human periodontal ligament fibroblasts (HPDLFs). An extensive search was performed in major electronic databases including PubMed (1997), EMBASE (1947) and Web of Science (1956) and supplemented by hand search of reference lists and relevant laser journals for cell culture studies investigating the effect of diode low-level lasers on HGFs and HPDLFs published from January 1995 to December 2015. A total of 21 studies were included after screening 324 independent records, amongst which eight targeted HPDLFs and 13 focussed on HGFs. The diode low-level laser showed positive effects on promoting fibroblast proliferation and osteogenic differentiation and modulating cellular inflammation via changes in gene expression and the release of growth factors, bone-remodelling markers or inflammatory mediators in a parameter-dependent manner. Repeated irradiations with wavelengths in the red and near-infrared range and at an energy density below 16 J/cm2 elicited favourable responses. However, considerable variations and weaknesses in the study designs and laser protocols limited the interstudy comparison and clinical transition. Current evidence showed that diode low-level lasers with adequate parameters stimulated the proliferation and modulated the inflammation of fibroblasts derived from human periodontal tissue. However, further in vitro studies with better designs and more appropriate study models and laser parameters are anticipated to provide sound evidence for clinical studies and practice.
Similar content being viewed by others
References
Sun G, Tunér J (2004) Low-level laser therapy in dentistry. Dent Clin N Am 48:1061–1076. doi:10.1016/j.cden.2004.05.004
de Paula EC, de Freitas PM, Esteves-Oliveira M et al (2010) Laser phototherapy in the treatment of periodontal disease. A review. Lasers Med Sci 25:781–792. doi:10.1007/s10103-010-0812-y
Ge MK, He WL, Chen J et al (2015) Efficacy of low-level laser therapy for accelerating tooth movement during orthodontic treatment: a systematic review and meta-analysis. Lasers Med Sci 30:1609–1618. doi:10.1007/s10103-014-1538-z
Ren C, McGrath C, Yang Y (2015) The effectiveness of low-level diode laser therapy on orthodontic pain management: a systematic review and meta-analysis. Lasers Med Sci 30:1881–1893. doi:10.1007/s10103-015-1743-4
Tuner J, Hode L (2007) The laser therapy handbook: a guide for research scientists, doctors, dentists, veterinarians and other interested parties within the medical field. Prima Books, Grangesberg
Houreld NN, Abrahamse H (2008) Laser light influences cellular viability and proliferation in diabetic-wounded fibroblast cells in a dose-and wavelength-dependent manner. Lasers Med Sci 23:11–18
Pinheiro AL, Gerbi ME (2006) Photoengineering of bone repair processes. Photomed Laser Surg 24:169–178
Huang YY, Chen AC, Carroll JD, Hamblin MR (2009) Biphasic dose response in low level light therapy. Dose–response 7:358–383. doi:10.2203/dose-response.09-027.Hamblin
Posten W, Wrone DA, Dover JS, Arndt KA, Silapunt S, Alam M (2005) Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg 31:334–340
Bjordal JM, Johnson MI, Iversen V, Aimbire F, Lopes-Martins RA (2006) Low-level laser therapy in acute pain: a systematic review of possible mechanisms of action and clinical effects in randomized placebo-controlled trials. Photomed Laser Surg 24:158–168
Gao X, Xing D (2009) Molecular mechanisms of cell proliferation induced by low power laser irradiation. J Biomed Sci 16:1–16. doi:10.1186/1423-0127-16-4
Weinreb M, Nemcovsky CE (2015) In vitro models for evaluation of periodontal wound healing/regeneration. Periodontol 2000 68:41–54. doi:10.1111/prd.12079
Giannopoulou C, Cimasoni G (1996) Functional characteristics of gingival and periodontal ligament fibroblasts. J Dent Res 75:895–902
Beertsen W, McCulloch CA, Sodek J (1997) The periodontal ligament: a unique, multifunctional connective tissue. Periodontol 2000 13:20–40
Peplow PV, Chung TY, Baxter GD (2010) Laser photobiomodulation of proliferation of cells in culture: a review of human and animal studies. Photomed Laser Surg 28(S1):S3–S40. doi:10.1089/pho.2010.2771
Peplow PV, Chung TY, Ryan B, Baxter GD (2011) Laser photobiomodulation of gene expression and release of growth factors and cytokines from cells in culture: a review of human and animal studies. Photomed Laser Surg 29:285–304. doi:10.1089/pho.2010.2846
Choi EJ, Yim JY, Koo KT et al (2010) Biological effects of a semiconductor diode laser on human periodontal ligament fibroblasts. J Periodontal Implant Sci 40:105–110. doi:10.5051/jpis.2010.40.3.105
Kreisler M, Christoffers AB, Willershausen B, d’Hoedt B (2003) Effect of low‐level GaAlAs laser irradiation on the proliferation rate of human periodontal ligament fibroblasts: an in vitro study. J Clin Periodontol 30:353–358
Wu JY, Chen CH, Yeh LY, Yeh ML, Ting CC, Wang YH (2013) Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate. Int J Oral Sci 5:85–91. doi:10.1038/ijos.2013.38
Mayahara K, Yamaguchi A, Sakaguchi M, Igarashi Y, Shimizu N (2010) Effect of Ga‐Al‐As laser irradiation on COX‐2 and cPLA2‐α and cPLA2 radiation on COX2010) effect of gas the proliferat. Lasers Surg Med 42:489–493. doi:10.1002/lsm.20871
Huang TH, Liu SL, Chen CL, Shie MY, Kao CT (2013) Low-level laser effects on simulated orthodontic tension side periodontal ligament cells. Photomed Laser Surg 31:72–77. doi:10.1089/pho.2012.3359
Ozawa Y, Shimizu N, Abiko Y (1997) Low‐energy diode laser irradiation reduced plasminogen activator activity in human periodontal ligament cells. Lasers Surg Med 21:456–463
Shimizu N, Yamaguchi M, Goseki T, Shibata Y, Takiguchi H, Iwasawa T, Abiko Y (1995) Inhibition of prostaglandin E2 and interleukin 1-β production by low-power laser irradiation in stretched human periodontal ligament cells. J Dent Res 74:1382–1388
Huang TH, Chen CC, Liu SL, Lu YC, Kao CT (2014) A low-level diode laser therapy reduces the lipopolysaccharide (LPS)-induced periodontal ligament cell inflammation. Laser Phys Lett. doi:10.1088/1612-2011/11/7/075602
Almeida-Lopes L, Rigau J, Zângaro RA, Guidugli-Neto J, Jaeger MM (2001) Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence. Lasers Surg Med 29:179–184
Marques MM, Pereira AN, Fujihara NA, Nogueira FN, Eduardo CP (2004) Effect of low-power laser irradiation on protein synthesis and ultrastructure of human gingival fibroblasts. Lasers Surg Med 34:260–265
Damante CA, De Micheli G, Miyagi SPH, Feist IS, Marques MM (2009) Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts. Lasers Med Sci 24:885–891. doi:10.1007/s10103-008-0582-y
Hakki SS, Bozkurt SB (2012) Effects of different setting of diode laser on the mRNA expression of growth factors and type I collagen of human gingival fibroblasts. Lasers Med Sci 27:325–331. doi:10.1007/s10103-010-0879-5
Basso FG, Pansani TN, Turrioni APS, Bagnato VS, Hebling J, de Souza Costa CA (2012) In vitro wound healing improvement by low-level laser therapy application in cultured gingival fibroblasts. Int J Dent. doi:10.1155/2012/719452
Azevedo LH, de Paula EF, Moreira MS, de Paula EC, Marques MM (2006) Influence of different power densities of LILT on cultured human fibroblast growth. Lasers Med Sci 21:86–89
Frozanfar A, Ramezani M, Rahpeyma A, Khajehahmadi S, Arbab HR (2013) The effects of low level laser therapy on the expression of collagen type I gene and proliferation of human gingival fibroblasts (Hgf3-Pi 53): in vitro study. Iran J Basic Med Sci 16:1071–1074
Saygun I, Karacay S, Serdar M, Ural AU, Sencimen M, Kurtis B (2008) Effects of laser irradiation on the release of basic fibroblast growth factor (bFGF), insulin like growth factor-1 (IGF-1), and receptor of IGF-1 (IGFBP3) from gingival fibroblasts. Lasers Med Sci 23:211–215
Kreisler M, Christoffers AB, Al-Haj H, Willershausen B, d’Hoedt B (2002) Low level 809-nm diode laser-induced in vitro stimulation of the proliferation of human gingival fibroblasts. Lasers Surg Med 30:365–369
Nomura K, Yamaguchi M, Abiko Y (2001) Inhibition of interleukin-1β production and gene expression in human gingival fibroblasts by low-energy laser irradiation. Lasers Med Sci 16:218–223
Sakurai Y, Yamaguchi M, Abiko Y (2000) Inhibitory effect of low-level laser irradiation on LPS-stimulated prostaglandin E2 production and cyclooxygenase-2 in human gingival fibroblasts. Eur J Oral Sci 108:29–34
Takema T, Yamaguchi M, Abiko Y (2000) Reduction of plasminogen activator activity stimulated by lipopolysaccharide from periodontal pathogen in human gingival fibroblasts by low-energy laser irradiation. Lasers Med Sci 15:35–42. doi:10.1007/s101030050045
Basso FG, Pansani TN, Soares DG, Scheffel DL, Bagnato VS, de Souza Costa CA, Hebling J (2015) Biomodulation of inflammatory cytokines related to oral mucositis by low-level laser therapy. Photochem Photobiol 91:952–956. doi:10.1111/php.12445
Brade H (1999) Endotoxin in health and disease. Marcel Dekker, New York
Erac Y, Selli C, Filik P, Tosun M (2014) Effects of passage number on proliferation and store-operated calcium entry in A7r5 vascular smooth muscle cells. J Pharmacol Toxicol Methods 70:1–5. doi:10.1016/j.vascn.2014.03.001
Hughes P, Marshall D, Reid Y, Parkes H, Gelber C (2007) The costs of using unauthenticated, over-passaged cell lines: how much more data do we need? Biotechniques 43:575–586. doi:10.2144/000112598
Pirkmajer S, Chibalin AV (2011) Serum starvation: caveat emptor. Am J Physiol Cell Physiol 301:C272–C279. doi:10.1152/ajpcell.00091.2011
Tran Hle B, Doan VN, Le HT, Ngo LT (2014) Various methods for isolation of multipotent human periodontal ligament cells for regenerative medicine. In Vitro Cell Dev Biol Anim 50:597–602. doi:10.1007/s11626-014-9748-z
Yang L, Yang Y, Wang S, Li Y, Zhao Z (2015) In vitro mechanical loading models for periodontal ligament cells: from two-dimensional to three-dimensional models. Arch Oral Biol 60:416–424. doi:10.1016/j.archoralbio.2014.11.012
Kapoor P, Kharbanda OP, Monga N, Miglani R, Kapila S (2014) Effect of orthodontic forces on cytokine and receptor levels in gingival crevicular fluid: a systematic review. Prog Orthod 15:65. doi:10.1186/s40510-014-0065-6
Calderín S, García-Núñez JA, Gómez C (2013) Short-term clinical and osteoimmunological effects of scaling and root planing complemented by simple or repeated laser phototherapy in chronic periodontitis. Lasers Med Sci 28:157–166. doi:10.1007/s10103-012-1104-5
Qadri T, Miranda L, Tunér J, Gustafsson A (2005) The short-term effects of low-level lasers as adjunct therapy in the treatment of periodontal inflammation. J Clin Periodontol 32:714–719
Bicakci AA, Kocoglu-Altan B, Toker H, Mutaf I, Sumer Z (2012) Efficiency of low-level laser therapy in reducing pain induced by orthodontic forces. Photomed Laser Surg 30:460–465. doi:10.1089/pho.2012.3245
Safavi SM, Kazemi B, Esmaeili M, Fallah A, Modarresi A, Mir M (2008) Effects of low-level He-Ne laser irradiation on the gene expression of IL-1beta, TNF-alpha, IFN-gamma, TGF-beta, bFGF, and PDGF in rat’s gingiva. Lasers Med Sci 23:331–335
Yoshida T, Yamaguchi M, Utsunomiya T et al (2009) Low-energy laser irradiation accelerates the velocity of tooth movement via stimulation of the alveolar bone remodeling. Orthod Craniofacial Res 12:289–298. doi:10.1111/j.1601-6343.2009.01464.x
Kawasaki K, Shimizu N (2000) Effects of low-energy laser irradiation on bone remodeling during experimental tooth movement in rats. Lasers Surg Med 26:282–291
Wu JY, Chen CH, Wang CZ, Ho ML, Yeh ML, Wang YH (2013) Low-power laser irradiation suppresses inflammatory response of human adipose-derived stem cells by modulating intracellular cyclic AMP level and NF-kB activity. PLoS One 8, e54067. doi:10.1371/journal.pone.0054067
Häkkinen L, Uitto VJ, Larjava H (2000) Cell biology of gingival wound healing. Periodontol 2000 24:127–152
Huang H, Williams RC, Kyrkanides S (2014) Accelerated orthodontic tooth movement: molecular mechanisms. Am J Orthod Dentofac Orthop 146:620–632. doi:10.1016/j.ajodo.2014.07.007
Yamamoto T, Kita M, Oseko F, Nakamura T, Imanishi J, Kanamura N (2006) Cytokine production in human periodontal ligament cells stimulated with Porphyromonas gingivalis. J Periodontal Res 41:554–559
Kasai K, Chou MY, Yamaguchi M (2015) Molecular effects of low-energy laser irradiation during orthodontic tooth movement. Semin Orthod 21:203–209
Acknowledgments
This research was supported by Health and Medical Research Fund of Hong Kong (01121056).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Ren, C., McGrath, C., Jin, L. et al. Effect of diode low-level lasers on fibroblasts derived from human periodontal tissue: a systematic review of in vitro studies. Lasers Med Sci 31, 1493–1510 (2016). https://doi.org/10.1007/s10103-016-2026-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10103-016-2026-4