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Influence of different power densities of LILT on cultured human fibroblast growth

A pilot study

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Abstract

The aim of this in vitro study was to analyze the influence of different power densities of low-intensity laser therapy (LILT) on the biomodulation of human gingival fibroblasts. The cells were cultured in nutritional deficit. Laser irradiation was carried out with a GaAlAs diode laser (λ-660 nm, 2 J/cm2). The irradiation was done twice with 12-h interval using the punctual technique, at continuous mode and in contact. The experimental groups were: I, control, nonirradiated; II, power of 10 mW and intensity≅142.85 mW/cm2; III, 29 mW and 428.57 mW/cm2. Growth curves were obtained by using the trypan blue dye exclusion assay. The cell growth of the irradiated groups was significantly higher than control group (P≤0.05). The cultures of Group II presented cell growth superior to that of Group III. Based on the conditions of this study, we concluded that the power density influences cell growth in an inversely proportional manner.

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Acknowledgements

We are grateful to LELO - FOUSP (Special Laboratory of Lasers in Dentistry - São Paulo University), and to FAPESP (Fundação de Apoio à Pesquisa do Estado de São Paulo) for the continuous support.

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Authors and Affiliations

  1. Department of Stomatology, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil

    Luciane Hiramatsu Azevedo & Fernanda de Paula Eduardo

  2. Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil

    Maria Stella Moreira, Carlos de Paula Eduardo & Márcia Martins Marques

  3. Faculdade de Odontologia, Departamento de Dentística, Av. Prof. Lineu Prestes, Universidade de São Paulo, 2227, Cidade Universitária, São Paulo, 05508 900, Brazil

    Márcia Martins Marques

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  1. Luciane Hiramatsu Azevedo
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  2. Fernanda de Paula Eduardo
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  3. Maria Stella Moreira
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  4. Carlos de Paula Eduardo
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  5. Márcia Martins Marques
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Correspondence to Márcia Martins Marques.

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Azevedo, L.H., de Paula Eduardo, F., Moreira, M.S. et al. Influence of different power densities of LILT on cultured human fibroblast growth. Lasers Med Sci 21, 86–89 (2006). https://doi.org/10.1007/s10103-006-0379-9

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  • DOI: https://doi.org/10.1007/s10103-006-0379-9

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