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The low level laser therapy (LLLT) operating in 660 nm reduce gene expression of inflammatory mediators in the experimental model of collagenase-induced rat tendinitis

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Abstract

Tendinopathy is a common disease with a variety of treatments and therapies. Laser therapy appears as an alternative treatment. Here, we investigate the effects of laser irradiation in an experimental model of tendinitis induced by collagenase injection on rats’ Achilles tendon, verifying its action in important inflammatory markers. Male Wistar rats were used and divided into five groups: control saline (C), non-treated tendinitis (NT) and tendinitis treated with sodium diclofenac (D) or laser (1 J) and (3 J). The tendinitis was induced by collagenase (100 μg/tendon) on the Achilles tendon, which was removed for further analyses. The gene expression for COX-2; TNF-α; IL-6; and IL-10 (RT-PCR) was measured. The laser irradiation (660 nm, 100 mW, 3 J) used in the treatment of the tendinitis induced by collagenase in Achilles tendon in rats was effective in the reduction of important pro-inflammatory markers such as IL-6 and TNF-α, becoming a promising tool for the treatment of tendon diseases.

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Authors and Affiliations

  1. Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University, São Paulo, 01504-001, Brazil

    Romildo Torres-Silva, Ernesto Cesar Pinto Leal-Junior & Rodrigo Labat Marcos

  2. Technology Research - NPT, UMC, Mogi das Cruzes, 08780-911, Brazil

    Rodrigo Alvaro Brandão Lopes-Martins & Rodney Pallotta

  3. Department of Global Health, University of Bergen, Bergen, Norway

    Jan Magnus Bjordal

  4. Departamento de Ciências da Saúde, Universidade Cruzeiro do Sul, São Paulo, Brazil

    Lucio Frigo

  5. Laboratoire d′Energétique et de Mécanique Théorique et Appliquée (LEMTA), UMR 7563 CNRS, Université de Lorraine (UL), 54504, Vandœuvre-lès-Nancy, France

    Rachid Rahouadj

  6. Laboratoire Physique et Mécanique Textiles (LPMT), Université de Haute Alsace (UHA), F-68093, Mulhouse, France

    Gilles Arnold

  7. Université de Strasbourg (UdS), ICube, UMR 7357 CNRS, 67411, Illkirch, France

    Gilles Arnold

  8. Ingénierie Moléculaire, Physiopathologie Articulaire (IMoPA), UMR 7365 CNRS, Université de Lorraine (UL), 54505, Vandœuvre-lès-Nancy, France

    Jacques Magdalou

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  1. Romildo Torres-Silva
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  10. Rodrigo Labat Marcos
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Correspondence to Rodrigo Labat Marcos.

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Torres-Silva, R., Lopes-Martins, R.A.B., Bjordal, J.M. et al. The low level laser therapy (LLLT) operating in 660 nm reduce gene expression of inflammatory mediators in the experimental model of collagenase-induced rat tendinitis. Lasers Med Sci 30, 1985–1990 (2015). https://doi.org/10.1007/s10103-014-1676-3

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  • DOI: https://doi.org/10.1007/s10103-014-1676-3

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