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Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells

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Abstract

The aim of this study was to investigate the osteoblastic activity of cells derived from the midpalatal suture upon treatment with low-level laser therapy (LLLT) after rapid maxillary expansion (RME). A total of 30 rats were divided into two groups: experimental I (15 rats with RME without LLLT) and experimental II (15 rats with RME + LLLT). The rats were euthanized at 24 h, 48 h, and 7 days after RME, when the osteoblastic cells derived from the rats’ midpalatal suture were explanted. These cells were cultured for periods up to 17 days, and then in vitro osteogenesis parameters and gene expression markers were evaluated. The cellular doubling time in the proliferative stage (3–7 days) was decreased in cultured cells harvested from the midpalatal suture at 24 and 48 h after RME + LLLT, as indicated by the increased growth of the cells in a culture. Alkaline phosphatase activity at days 7 and 14 of the culture was increased by LLLT in cells explanted from the midpalatal suture at 24 and 48 h and 7 days after RME. The mineralization at day 17 was increased by LLLT after RME in all periods. Results from the real-time PCR demonstrated that cells harvested from the LLLT after RME group showed higher levels of ALP, Runx2, osteocalcin, type I collagen, and bone sialoprotein mRNA than control cells. More pronounced effects on ALP activity, mineralization, and gene expression of bone markers were observed at 48 h after RME and LLLT. These results indicate that the LLLT applied after RME is able to increase the proliferation and the expression of an osteoblastic phenotype in cells derived from the midpalatal suture.

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Acknowledgments

We are deeply grateful to Roger Rodrigo Fernandes for excellent laboratory assistance at the Cell Culture Laboratory, School of Dentistry of Ribeirao Preto, University of São Paulo. We gratefully acknowledge the Brazilian agencies CAPES/FAPESP for financial support.

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No competing financial interests exist.

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Authors and Affiliations

  1. Department of Pediatric Dentistry and Othodontic, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Av do Cafe, s/n, 14040-904, Ribeirao Preto, SP, Brazil

    Ana Paula R. Bernardes da Silva, Adriana Sasso Stuani, Andrea Sasso Stuani & Maria Bernadete Sasso Stuani

  2. Cell Culture Laboratory, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, 14040-904, Brazil

    Alice D. Petri, Grasiele E. Crippa & Adalberto Luiz Rosa

Authors
  1. Ana Paula R. Bernardes da Silva
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  2. Alice D. Petri
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  3. Grasiele E. Crippa
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  4. Adriana Sasso Stuani
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  5. Andrea Sasso Stuani
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  6. Adalberto Luiz Rosa
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  7. Maria Bernadete Sasso Stuani
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Correspondence to Maria Bernadete Sasso Stuani.

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da Silva, A.P.R.B., Petri, A.D., Crippa, G.E. et al. Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells. Lasers Med Sci 27, 777–783 (2012). https://doi.org/10.1007/s10103-011-0968-0

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  • DOI: https://doi.org/10.1007/s10103-011-0968-0

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