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Laser and LED phototherapy on midpalatal suture after rapid maxilla expansion: Raman and histological analysis

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Abstract

The aim of this study was to analyze the effect of laser or LED phototherapy on the acceleration of bone formation at the midpalatal suture after rapid maxilla expansion. Forty-five rats were divided into groups at 7 days (control, expansion, expansion and laser irradiation, and expansion and LED irradiation) and into 14 days (expansion, expansion and laser in the 1st week, expansion and LED in the 1st week, expansion and laser in the 1st and 2nd weeks, expansion and LED in the 1st and 2nd weeks). Laser/LED irradiation occurred every 48 h. Expansion was accomplished with a spatula and maintained with a triple helicoid of 0.020-in stainless steel orthodontic wire. A diode laser (λ780 nm, 70 mW, spot of 0.04 cm2, t = 257 s, SAEF of 18 J/cm2) or a LED (λ850 ± 10 nm, 150 ± 10 mW, spot of 0.5 cm2, t = 120 s, SAEF of 18 J/cm2) was applied in one point in the midpalatal suture immediately behind the upper incisors. Raman spectroscopy and histological analyses of the suture region were carried and data was submitted to statistical analyses (p ≤ 0.05). Raman spectrum analysis demonstrated that irradiation increases hydroxyapatite in the midpalatal suture after expansion. In the histological analysis of various inflammation, there was a higher production of collagen and osteoblastic activity and less osteoclastic activity. The results showed that LED irradiation associated to rapid maxillary expansion improves bone repair and could be an alternative to the use of laser in accelerating bone formation in the midpalatal suture.

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References

  1. Saito S, Shimizu N (1997) Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat. Am J Orthod Dentofac Orthop 111:525–532

    Article  CAS  Google Scholar 

  2. Braun S, Bottrel JA, Lee K et al (2000) The biomechanics of rapid maxillary sutural expansion. Am J Orthod Dentofac Orthop 118:257–261

    Article  CAS  Google Scholar 

  3. Marini I, Bonetti GA, Achilli V, Salemi G (2007) A photogrammetric technique for the analysis of palatal three-dimensional changes during rapid maxillary expansion. Eur J Orthod 29:26–30

    Article  PubMed  Google Scholar 

  4. Desmet KD, Paz DA, Correy JJ (2006) Clinical and experimental applications of NIR-LED photobiomodulation. Photomed Laser Surg 24:121–128

    Article  CAS  PubMed  Google Scholar 

  5. Pinheiro ALB, Santos NRS, Oliveira PC et al (2013) The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with miniplates: a Raman spectral study on rabbits. Lasers Med Sci 28:513–518

    Article  PubMed  Google Scholar 

  6. Pinheiro ALB, Gerbi MEMM (2006) Photoengineering of bone repair processes. Photomed Laser Surg 24:169–178

    Article  CAS  PubMed  Google Scholar 

  7. Merli LAS, Santos MTBR, Genovese WJ, Faloppa F (2005) Effect of low-intensity laser irradiation on the process of bone repair. Photomed Laser Surg 23:212–215

    Article  PubMed  Google Scholar 

  8. Habib FAL, Gama SKC, Ramalho LMP, Cangussú MC, dos Santos Neto FP, Lacerda JA, de Araújo TM, Pinheiro AL (2012) Effect of laser phototherapy on the hyalinization following orthodontic tooth movement in rats. Photomed Laser Surg 30:179–185

    Article  PubMed  Google Scholar 

  9. Habib FAL, Gama SKC, Ramalho LMP, Cangussú MC, Santos Neto FP, Lacerda JA, Araújo TM, Pinheiro AL (2010) Laser-induced alveolar bone changes during orthodontic movement: a histological study on rodents. Photomed Laser Surg 28:823–830

    Article  CAS  PubMed  Google Scholar 

  10. Pinheiro ALB, Soares LGP, Cangussu MCT, Santos NR, Barbosa AF, Silveira Júnior L (2012) Effects of LED phototherapy on bone defects grafted with MTA, bone morphogenetic proteins and guided bone regeneration: a Raman spectroscopic study. Lasers Med Sci 5:903–916

    Article  Google Scholar 

  11. Pinheiro ALB, Soares LG, Barbosa AF, Ramalho LM, dos Santos JN (2012) Does LED phototherapy influence the repair of bone defects grafted with MTA, bone morphogenetic proteins, and guided bone regeneration? A description of the repair process on rodents. Lasers Med Sci 27:1013–1024

    Article  PubMed  Google Scholar 

  12. Oliveira Sampaio SC, de C Monteiro JS, Cangussú MC, Pires Santos GM, dos Santos MA, dos Santos JN, Pinheiro AL (2013) Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers Med 28:799–806

    Article  Google Scholar 

  13. Souza APC, Neto AAPAV, Marchionni AMT, de Araújo Ramos M, dos Reis JA Jr, Pereira MC, Cangussú MC, de Almeida Reis SR, Pinheiro AL (2011) Effect of LED phototherapy (l70020 nm) on TGF-b expression during wound healing: an immunohistochemical study in a rodent model. Photomed Laser Surg 29:605–6011

    Article  Google Scholar 

  14. Pinheiro ALB et al (2011) Light microscopic description of the effects of laser phototherapy on bone defects grafted with mineral trioxide aggregate, bone morphogenetic proteins, and guided bone regeneration in a rodent model. J Biomed Mater Res 98A:212–221

    Article  CAS  Google Scholar 

  15. Posten W et al (2005) Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg 31:334–340

    Article  CAS  PubMed  Google Scholar 

  16. Sampaio SCP et al (2013) Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers Med 28:799–806

    Article  Google Scholar 

  17. Tachiahra R, Farinelli WA, Anderson R (2002) Low intensity light-induced vasodilation in vivo. Lasers Surg Med 30:11

    Article  Google Scholar 

  18. Hanlon EB et al (2000) Prospects for in vivo Raman spectroscopy. Phys Med Biol 45:R1–R59

    Article  CAS  PubMed  Google Scholar 

  19. Paschalis EP, Mendelsohn R, Boskey AL (2011) Infrared assessment of bone quality. Clin Orthop Relat Res 469:2170–2178

    Article  PubMed  PubMed Central  Google Scholar 

  20. Incerti Parenti S, Panseri S, Gracco A, Sandri M, Tampieri A, Alessandri Bonetti G (2013) Effect of low-level laser irradiation on osteoblast-like cells cultured on porous hydroxyapatite scaffolds. Ann Ist Super Sanita 49(3):255–260

    PubMed  Google Scholar 

  21. Stein A, Benayahu D, Maltz L, Oron U (2005) Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 23:161–166

    Article  CAS  PubMed  Google Scholar 

  22. Lopes CB, Pacheco MTT, Silveira Junior L, Cangussú MC, Pinheiro AL (2010) The effect of the association of near infrared laser therapy, bone morphogenetic proteins, and guided bone regeneration on tibial fractures treated with internal rigid fixation: a Raman spectroscopic study. J Biomed Mater Res 94:1257–1263

    Google Scholar 

  23. Lopes CB, Pinheiro ALB, Sathaiah S, Duarte J, Cristinamartins M (2005) Infrared laser light reduces loading time of dental implants: a Raman spectroscopic study. Photomed Laser Surg 23:27–31

    Article  CAS  PubMed  Google Scholar 

  24. Ma J, Wu Y, Zhang W, Smales RJ, Huang Y, Pan Y, Wang L (2008) Up-regulation of multiple proteins and biological processes during maxillary expansion in rats. BMC Musculoskelet Disord 9:1–11

    Article  Google Scholar 

  25. Silva APRB, Petri AD, Crippa GE, Petri AD, Crippa GE, Stuani AS, Stuani AS, Rosa AL, Stuani MB (2012) Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells. Lasers Med Sci 27:777–783

    Article  PubMed  Google Scholar 

  26. Ekizer A, Uysal T, Guray E, Yuksel Y (2013) Light emitting diodes photomodulation: effect on bone formation in orthopedically expanded suture in rats early bone changes. Lasers Med Sci 28(5):1263–1270

    Article  PubMed  Google Scholar 

  27. Hou B, Fukai N, Olsen BR (2007) Mechanical force-induced midpalatal suture remodeling in mice. Bone 40:1483–1493

    Article  PubMed  PubMed Central  Google Scholar 

  28. Huang PJ, Huang YC, Su MF, Yang TY, Huang JR, Jiang CP (2007) In vitro observations on the influence of copper peptide aids for the LED photoirradiation of fibroblast collagen synthesis. Photomed Laser Surg 25:183–190

    Article  CAS  PubMed  Google Scholar 

  29. Morris MD, Mandair GS (2011) Raman assessment of bone quality. Clin Orthop Relat Res 469:2160–2169

    Article  PubMed  Google Scholar 

  30. Baxter GD (1994) Bioenergetics and tissue optics. In: Therapeutic lasers theory and practice, 1st edn. Churchill Livingstone, New York, pp 67–88

    Google Scholar 

Download references

Acknowledgments

We would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for providing financial support for this project.

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Authors and Affiliations

  1. Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil

    Cristiane Becher Rosa, Fernando Antonio Lima Habib, Jean Nunes dos Santos, Maria Cristina T. Cangussu, Artur Felipe Santos Barbosa, Isabele Cardoso Vieira de Castro & Antônio Luiz Barbosa Pinheiro

  2. Orthodontics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil

    Telma Martins de Araújo

  3. National Institute of Optics and Photonics, Physics Institute of São Carlos, University of São Paulo, São Carlos, SP, 13560-970, Brazil

    Antônio Luiz Barbosa Pinheiro

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  1. Cristiane Becher Rosa
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  2. Fernando Antonio Lima Habib
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  3. Telma Martins de Araújo
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  4. Jean Nunes dos Santos
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  5. Maria Cristina T. Cangussu
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  6. Artur Felipe Santos Barbosa
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  7. Isabele Cardoso Vieira de Castro
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  8. Antônio Luiz Barbosa Pinheiro
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Correspondence to Antônio Luiz Barbosa Pinheiro.

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The authors received a grant from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), a government research agency, but have full control of all primary data and agree to allow the journal to review their data if requested.

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Rosa, C.B., Habib, F.A.L., de Araújo, T.M. et al. Laser and LED phototherapy on midpalatal suture after rapid maxilla expansion: Raman and histological analysis. Lasers Med Sci 32, 263–274 (2017). https://doi.org/10.1007/s10103-016-2108-3

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  • DOI: https://doi.org/10.1007/s10103-016-2108-3

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