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Inhibitory effects of Orostachys malacophyllus var. iwarenge extracts on reactive oxygen species production and lipid accumulation during 3T3-L1 adipocyte differentiation

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Abstract

Orostachys malacophyllus var. iwarenge was extracted with water, 30 and 70% ethyl alcohol. The ethyl alcohol extracts showed higher total phenol contents and greater antioxidant effects than the water extract. Treatment with the 70% alcohol extract inhibited reactive oxygen species (ROS) production and lipid accumulation during 3T3-L1 adipogenesis. Furthermore, the 70% extract inhibited the mRNA and protein expressions of the pro-oxidant enzyme NADPH oxidase 4 and of the NADPH-producing enzyme glucose-6-phosphate dehydrogenase. The mRNA and protein expressions of antioxidant enzymes, such as copper-zinc superoxide dismutase and manganese superoxide dismutase increased in cells treated with the 70% alcohol extract. In addition, this extract suppressed the mRNA and protein levels of adipogenic transcription factors and of their marker genes. These results indicate that O. malacophyllus extracts inhibit lipid accumulation and ROS production by controlling adipogenic factors and pro-/anti-oxidant enzyme responses.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea Government (No. 2016R1A2B4014740).

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Author notes

  1. Miran Jang and Hye-young Choi have equally contributed to this research as co-first authors.

Authors and Affiliations

  1. Plant Resources Research Institute, Duksung Women’s University, Seoul, 132-714, Korea

    Miran Jang, Hye-young Choi & Gun-Hee Kim

  2. Department of Food and Nutrition, Duksung Women’s University, 419, Ssangmun-Dong, Dobong-Gu, Seoul, 132-714, Korea

    Miran Jang, Hye-young Choi & Gun-Hee Kim

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  1. Miran Jang
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  2. Hye-young Choi
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  3. Gun-Hee Kim
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Correspondence to Gun-Hee Kim.

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Jang, M., Choi, Hy. & Kim, GH. Inhibitory effects of Orostachys malacophyllus var. iwarenge extracts on reactive oxygen species production and lipid accumulation during 3T3-L1 adipocyte differentiation. Food Sci Biotechnol 28, 227–236 (2019). https://doi.org/10.1007/s10068-018-0426-x

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  • DOI: https://doi.org/10.1007/s10068-018-0426-x

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