Skip to main content
Log in

Quantitative determination of conjugated linoleic acids in hydrogenated vegetable oils using refractive index

  • Research Note
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Conjugated linoleic acid contents in hydrogenated vegetable oils were differentially determined using refractive indices when the iodine value could not be used. The refractive indices of soybean oil, cottonseed oil, and corn oil varied linearly with changes of linoleic acid contents of individual oils with determination coefficients of 0.91, 0.98, and 0.98, respectively. The refractive index can be used as a simple and fast method for control of the hydrogenation process of vegetable oils to obtain a desired conjugated linoleic acid content without fatty acid compositional analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Coenen JW. Hydrogenation of edible oils. J. Am. Oil Chem. Soc. 53: 382–389 (1976)

    Article  CAS  Google Scholar 

  2. Karabulut I, Kayahan M, Yaprak S. Determination of changes in some physical and chemical properties of soybean oil during hydrogenation. Food Chem. 81: 453–456 (2003)

    Article  CAS  Google Scholar 

  3. Ip C, Briggs SP, Haegele AD, Thompson HJ, Storkson J, Scimeca JA. The efficiency of conjugated linoleic acid in mammary cancer prevention is independent of the level or type of fat in the diet. Carcinogenesi. 17: 1045–1050 (1996)

    Article  CAS  Google Scholar 

  4. Jung MY, Choi NJ, Oh CH, Shin HK, Yoon SH. Selectively hydrogenated soybean oil exerts strong anti-prostate cancer activities. Lipid. 46: 287–295 (2011)

    Article  CAS  Google Scholar 

  5. Lee KN, Kritchevsky D, Pariza MW. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosi. 108: 19–25 (1994)

    Article  CAS  Google Scholar 

  6. Houseknecht KL, Vanden Heuvel JP, Moya-Camarena SY, Portocarrero CP, Peck LW, Nickel KP, Belury MA. Dietary conjugated linoleic acid normalizes impaired glucose tolerance in the Zucker Diabetic Fatty fa/fa Rat. Biochem. Bioph. Res. Co. 244: 678–682 (1998)

    Article  CAS  Google Scholar 

  7. Cook ME, Miller CC, Park Y, Pariza MW. Immune modulation by altered nutrient metabolism: Nutritional control of immune-induced growth depression. Poultry Sci. 72: 1301–1305 (1993)

    Article  CAS  Google Scholar 

  8. Park Y, Storkson JM, Albright KJ, Liu W, Pariza MW. Evidence that the trans-10, cis-12 isomer of conjugated linoleic acid induces body composition changes in mice. Lipid. 34: 235–241 (1999)

    Article  CAS  Google Scholar 

  9. Riserus U, Berglund L, Vessby B. Conjugated linoleic acid (CLA) reduced abdominal adipose tissue in obese middle-aged men with signs of metabolic syndrome: A randomized controlled trial. Int. J. Obesit. 25: 1129–1135 (2001)

    Article  CAS  Google Scholar 

  10. Choi NJ, Kwon D, Yun SH, Jung MY, Shin HK. Selectively hydrogenated soybean oil with conjugated linoleic acid modifies body composition and plasma lipids in rats. J. Nutr. Biochem. 15: 411–417 (2004)

    Article  CAS  Google Scholar 

  11. Ha YL, Grim NK, Pariza MW. Newly Recognized Anticarcinogenic Fatty Acids: Identification and quantification in natural and processed cheeses. J. Agr. Food Chem. 37: 75–81 (1989)

    Article  CAS  Google Scholar 

  12. Chin SF, Liu W, Storkson JM, Ha YL, Pariza MW. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J. Food Compos. Anal. 5: 185–197 (1992)

    Article  CAS  Google Scholar 

  13. Banni S, Day BW, Evans RW, Corongiu FP. Lombardi B. Liquid chromatographicmass spectrometric analysis of conjugated diene fatty acids in a partially hydrogenated fat. J. Am. Oil Chem. Soc. 71: 1321–1325 (1994)

    Article  CAS  Google Scholar 

  14. Jung MY, Ha YL. Conjugated linoleic acid isomers in partially hydrogenated soybean oil obtained during nonselective and selective hydrogenation processes. J. Agr. Food Chem. 47: 704–708 (1994)

    Article  Google Scholar 

  15. Jung MO, Yoon SH, Jung MY. Effects of temperature and agitation rate on the formation of conjugated linoleic acids in soybean oil during hydrogenation process. J. Agr. Food Chem. 49: 3010–3016 (2001)

    Article  CAS  Google Scholar 

  16. Jung MO, Ju JW, Choi DS, Yoon SH, Jung MY. CLA formation in oils during hydrogenation process as affected by catalyst types, catalyst contents, hydrogen pressure, and oil species. J. Am. Oil Chem. Soc. 79: 501–510 (2002)

    Article  CAS  Google Scholar 

  17. Ju JW, So WS, Kim JH, Bae BJ, Choi EN, Kwon YH, Chung IM, Yoon SH, Jung MY. Effects of alcohol type and amounts on conjugated linoleic acid formation during catalytic transfer hydrogenation of soybean oil. J. Food Sci. 68: 1915–1922 (2003)

    Article  CAS  Google Scholar 

  18. Okkerse C, De Jonge A, Coenen JWE, Rozendaal A. Selective hydrogenation of soybean oil in the presence of copper catalysts. J. Am. Oil Chem. Soc. 44: 152–156 (1967)

    Article  CAS  Google Scholar 

  19. Mossoba MM, McDonald RE, Armstrong DJ, Page SW. Identification of minor C18 triene and conjugated diene isomers in hydrogenated soybean oil and margarine by GC-MI-FT-IR spectroscopy. J. Chromatogr. Sci. 29: 324–330 (1991)

    Article  CAS  Google Scholar 

  20. Cole CF, Hill GM, Adams AJ. Automated refractive index measurement of catalyst-laden edible oils undergoing partial hydrogenation. J. Am. Oil Chem. Soc. 71: 1339–1342 (1994)

    Article  CAS  Google Scholar 

  21. Majors KR, Milner RT. Relation between the iodine number and refractive index of crude soybean oil. Oil Soa. 16: 228–231 (1939)

    Article  CAS  Google Scholar 

  22. Athavale VT, Jatkar SKK. Hydrogenation of oils by the continuous hydrogenation of oils. J. Indian I. Sci. 21: 321–330 (2013)

    Google Scholar 

  23. Mattil KF, Longenecker HE. The use of refractive index measurements in fatty acid ester analyses. J. Am. Oil Chem. Soc. 21: 16–19 (1944)

    CAS  Google Scholar 

  24. AOCS. Official Methods and Recommended Practices of the AOCS. 6th ed. Method Cd 1c-85, Tp 1a-64. American Oil Chemists’ Society, Champaign, IL, USA (2009)

  25. Gunstone FD, Harwood JL, Dijkstra AJ. The lipid handbook. 3rd ed. CRC Press, Boca Raton, FL, USA. pp. 66–67 (2007)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Suk Hoo Yoon.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jung, M.Y., Park, J.S. & Yoon, S.H. Quantitative determination of conjugated linoleic acids in hydrogenated vegetable oils using refractive index. Food Sci Biotechnol 25, 121–124 (2016). https://doi.org/10.1007/s10068-016-0018-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-016-0018-6

Keywords

Navigation