Skip to main content
Log in

Optimization of various extraction methods for quercetin from onion skin using response surface methodology

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Quercertin and typical flavonoids present in onion skin draw interest due to bioactive properties. For utilizing quercetin from onion skin, conventional solvent extraction (CSE), microwave assisted extraction (MAE), and ultrasound assisted extraction (UAE) were employed. Statistic models of each method were proposed to estimate the best possible yield of quercertin employing response surface methodology (RSM). The effects of several independent variables including concentration of ethanol, provided power or the temperature, and reaction time were investigated. From 1 g sample of dried onion skin, the highest yield of each method could be achieved at 16.5 min of process time under 59.2°C for CSE with 59.3% ethanol, 117 s for MAE with 69.7% ethanol, and 21.7 min for UAE using power of 606.4 W with 43.8% ethanol. The most productive method was MAE, whose maximum yield was 20.3 and 30.8% higher than UAE and CSE, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, van Leeuwen PA. Flavonoids: A review of probable mechanisms of action and potential applications. Am. J. Clin. Nutr. 74: 418–425 (2001)

    CAS  Google Scholar 

  2. Lemańska K, van der Woude H, Szymusiak H, Boersma MG, Gliszczyńska-Świgło A, Rietjens IMCM, Tyrakowska B. The effect of catechol O-methylation on radical scavenging characteristics of quercetin and luteolin — A mechanistic insight. Free Radical Res. 38: 639–647 (2004)

    Article  Google Scholar 

  3. Leighton T, Ginther C, Fluss L, Harter WK, Cansado J, Notario V. Molecular characterization of quercetin and quercetin glycosides in Allium vegetables. pp. 220–238. In: Phenolic Compounds in Food and Their Effects on Health II. Huang MT, Ho CT, Lee CY (eds). American Chemical Society, Washington, DC, USA (1992)

    Chapter  Google Scholar 

  4. Williamson G, Manach C. Bioavailability and bioefficacy of polyphenols in humans. II. Review of 93 intervention studies. Am. J. Clin. Nutr. 81: 243S–255S (2005)

    CAS  Google Scholar 

  5. Chemat S, Lagha A, AitAmar H, Bartels PV, Chemat F. Comparison of conventional and ultrasound-assisted extraction of carvone and limonene from caraway seeds. Flavour Frag. J. 19: 188–195 (2004)

    Article  CAS  Google Scholar 

  6. Brachet A, Christen P, Veuthey JL. Focused microwave-assisted extraction of cocaine and benzoylecgonine from coca leaves. Phytochem. Analysis 13: 162–169 (2002)

    Article  CAS  Google Scholar 

  7. Toma M, Vinatoru M, Paniwnyk L, Mason TJ. Investigation of the effects of ultrasound on vegetal tissues during solvent extraction. Ultrason. Sonochem. 8: 137–142 (2001)

    Article  CAS  Google Scholar 

  8. Csiktusnádi Kiss GA, Forgács E, Cserháti T, Mota T, Morais H, Ramos A. Optimisation of the microwave-assisted extraction of pigments from paprika (Capsicum annuum L.) powders. J. Chromatogr. A 889: 41–49 (2000)

    Article  Google Scholar 

  9. Vinatoru M, Toma M, Radu O, Filip PI, Lazurca D, Mason TJ. The use of ultrasound for the extraction of bioactive principles from plant materials. Ultrason. Sonochem. 4: 135–139 (1997)

    Article  CAS  Google Scholar 

  10. Kwon YJ, Kwon JH, Kim HK. Oleoresin content and functional properties of fresh onion by microwave-assisted extraction. J. Korean Soc. Food Sci. Nutr. 28: 876–881 (1999)

    Google Scholar 

  11. Wang S, Chen F, Wu J, Wang Z, Liao X, Hu X. Optimization of pectin extraction assisted by microwave from apple pomace using response surface methodology. J. Food Eng. 78: 693–700 (2007)

    Article  CAS  Google Scholar 

  12. Wang L, Weller CL. Recent advances in extraction of nutraceuticals from plants. Trends Food Sci. Tech. 17: 300–312 (2006)

    Article  CAS  Google Scholar 

  13. Kaufmann B, Christen P, Veuthey J-L. Parameters affecting microwave-assisted extraction of withanolides. Phytochem. Analysis 12: 327–331 (2001)

    Article  CAS  Google Scholar 

  14. Yang Y, Zhang F. Ultrasound-assisted extraction of rutin and quercetin from Euonymus alatus (Thunb.) Sieb. Ultrason. Sonochem. 15: 308–313 (2008)

    Article  CAS  Google Scholar 

  15. Mason TJ, Paniwnyk L, Lorimer JP. The uses of ultrasound in food technology. Ultrason. Sonochem. 3: S253–S260 (1996)

    Article  CAS  Google Scholar 

  16. Madamba PS. The response surface methodology: An application to optimize dehydration operations of selected agricultural crops. Lebensm. -Wiss. Technol. 35: 584–592 (2002)

    Article  CAS  Google Scholar 

  17. Herrmann K. On the occurrence of flavonol and flavone glycosides in vegetables. Z. Lebensm. Unters. F. A. 186: 1–5 (1988)

    Article  CAS  Google Scholar 

  18. Crozier A, Lean MEJ, McDonald MS, Black C. Quantitative analysis of the flavonoid content of commercial tomatoes, onions, lettuce, and celery. J. Agr. Food Chem. 45: 590–595 (1997)

    Article  CAS  Google Scholar 

  19. Kang SK, Kim YD, Hyun KH, Kim YW, Seo JS, Park YK. Development of separating techniques on quercetin — related substances in onion (Allium cepa L.) — 2. Optimal extracting condition of quercetin — related substances in onion. J. Korean. Soc. Food Sci. Nutr. 27: 687–692 (1998)

    CAS  Google Scholar 

  20. Kiassos E, Mylonaki S, Makris DP, Kefalas P. Implementation of response surface methodology to optimise extraction of onion (Allium cepa) solid waste phenolics. Innov. Food Sci Emerg. 10: 246–252 (2009)

    Article  CAS  Google Scholar 

  21. Turner C, Turner P, Jacobson G, Almgren K, Waldeback M, Sjoberg P, Karlsson EN, Markides KE. Subcritical water extraction and β-glucosidase-catalyzed hydrolysis of quercetin glycosides in onion waste. Green Chem. 8: 949–959 (2006)

    Article  CAS  Google Scholar 

  22. Khiari Z, Makris D, Kefalas P. An investigation on the recovery of antioxidant phenolics from onion solid wastes employing water/ethanol-based solvent systems. Food Bioprocess Tech. 2: 337–343 (2009)

    Article  CAS  Google Scholar 

  23. Kim SJ, Kim GH. Quantification of quercetin in different parts of onion and its DPPH radical scavenging and antibacterial activity. Food Sci. Biotechnol. 15: 39–43 (2006)

    CAS  Google Scholar 

  24. Kurozawa LE, Park KJ, Hubinger MD. Optimization of the enzymatic hydrolysis of chicken meat using response surface methodology. J. Food Sci. 73: C405–C412 (2008)

    Article  CAS  Google Scholar 

  25. Jin EY, Park YS, Jang JK, Chung MS, Park H, Shim KS, Choi YJ. Extraction of quercetin and its glucosides from onion edible part using solvent extraction and various extraction assisting methods. Food Eng. Progress 13: 147–153 (2009)

    Google Scholar 

  26. Myers RH, Montgomery DC. Response Surface Methodology: Process and Product Optimization Using Designed Experiment. John Wiley & Sons Inc., New York, NY, USA (2002)

    Google Scholar 

  27. Lianfu Z, Zelong L. Optimization and comparison of ultrasound/microwave assisted extraction (UMAE) and ultrasonic assisted extraction (UAE) of lycopene from tomatoes. Ultrason. Sonochem. 15: 731–737 (2008)

    Article  Google Scholar 

  28. Vinatoru M. An overview of the ultrasonically assisted extraction of bioactive principles from herbs. Ultrason. Sonochem. 8: 303–313 (2001)

    Article  CAS  Google Scholar 

  29. Sun Y, Wang Z, Wu J, Chen F, Liao X, Hu X. Optimising enzymatic maceration in pretreatment of carrot juice concentrate by response surface methodology. Int. J. Food Sci. Tech. 41: 1082–1089 (2006)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Young Jin Choi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jin, E.Y., Lim, S., Kim, S.o. et al. Optimization of various extraction methods for quercetin from onion skin using response surface methodology. Food Sci Biotechnol 20, 1727–1733 (2011). https://doi.org/10.1007/s10068-011-0238-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-011-0238-8

Keywords

Navigation