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Novel approach for extraction of grape skin antioxidants by accelerated solvent extraction: Box–Behnken design optimization

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Abstract

Grape skin can be considered as an excellent and inexpensive source of polyphenol antioxidant compounds. A high-efficiency accelerated solvent extraction (ASE) method was developed for antioxidant polyphenols from grape skin. A three-factors three-level Box-Behnken design by response surface methodology was employed for optimization of extraction parameters in terms of total phenolic content, total anthocyanins content and antioxidant activity. The optimized condition was ethanol concentration of 48.80%, temperature of 50.79 °C and extraction time of 14.82 min. Under these conditions, the highest yields of polyphenol, the total phenolic content (15.24 mg GAE/g), total anthocyanins content (346.68 mg CGE/100 g) in grape skin, were obtained with significant antioxidant properties by DPPH, ABTS and FRAP assays. Moreover, the extracts from various grape skins by ASE, possessed ten main antioxidant polyphenols with the highest concentration of p-hydroxybenzoic acid and malvidin-3-O-glucoside. Compared with conventional solvent extraction, ASE extracted more amounts of polyphenols, exhibited more extraction level with shorter time and higher reproducibility.

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Acknowledgements

We are grateful to the estate of Zijing Gege wineries (Jilin province, China) for providing the grape samples used in this study.

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Authors and Affiliations

  1. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, Liaoning, People’s Republic of China

    Jing Li & Minna Zhang

  2. School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang, 110016, Liaoning, People’s Republic of China

    Shuting Zhang & Baoshan Sun

  3. Pólo Dois Portos, Instituto National de Investigação Agrária e Veterinária, I.P., Quinta da Almoinha, 2565-191, Dois Portos, Portugal

    Baoshan Sun

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  1. Jing Li
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  2. Shuting Zhang
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  4. Baoshan Sun
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Correspondence to Shuting Zhang or Baoshan Sun.

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Li, J., Zhang, S., Zhang, M. et al. Novel approach for extraction of grape skin antioxidants by accelerated solvent extraction: Box–Behnken design optimization. J Food Sci Technol 56, 4879–4890 (2019). https://doi.org/10.1007/s13197-019-03958-5

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  • DOI: https://doi.org/10.1007/s13197-019-03958-5

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